Metanephrines in Urine - HPLC
Only 1 sample clean up column required
Reliable separation of interferences
3-Methoxytyramine
Metanephrine
Normetanephrine
Clinical relevance
The biogenic amines normetanephrine, metanephrine, and 3-methoxytyramine are the primary metabolites produced in catecholamine metabolism. They are important parameters in diagnosing certain tumours in the adrenal medulla such as pheochromocytomas, neuroblastomas, ganglioneuromas and melanoblastomas. They have to be determined in addition to adrenaline, noradrenaline, dopamine, VMA and HVA in order to lower the risk of false-positive results.
Product advantages
- Only 1 sample clean up column required
- Effective removal of interferences
- Combined catecholamine/metanephrine analysis in urine is possible
This kit enables a reliable routine HPLC determination of metanephrine, normetanephrine, and 3-methoxytyramine in urine. The conjugated metanephrines are reliably released using acid hydrolysis. The pH level required for solid phase extraction can be adjusted very easily using the colour change indicator by adding the neutralisation buffer. The chromatographic determination is run on an isocratic HPLC system with an electrochemical detector. Optimised column and mobile phase ensure the reliable separation of possible interfering substances.
Kit content
Mobile Phase, 1000 ml
Calibration Standard, 10 ml
Internal Standard, 10 ml
Neutralisation Buffer, 300 ml
Wash Buffer, 250 ml
Elution Buffer, 250 ml
Sample Clean Up Columns, 100 pcs.
| Number of Tests | 100 | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Limit of quantification | 5 µg/l | ||||||||||||||||||||
| Linearity | up to 5000 µg/l | ||||||||||||||||||||
| Recovery | ~ 85 % | ||||||||||||||||||||
| Intraassay | CV < 4 % | ||||||||||||||||||||
| Interassay | CV < 6 % | ||||||||||||||||||||
| Analysis Time | aproxx. 14 min | ||||||||||||||||||||
| Sample Preparation |
|
||||||||||||||||||||
| Sample Stability | At +2 to +8 °C at least 5 days. Aliquot and freeze for longer storage. | ||||||||||||||||||||
| Specimen | 24 h urine (containing 25 % HCl); spontaneous urine (refer the data to urine creatinine) | ||||||||||||||||||||
| Pre-analytic Treatment | Specimens
Stability of specimens
Aliquot and freeze for longer storage. |
||||||||||||||||||||
| Injection Volume | 20 µl | ||||||||||||||||||||
| Flow rate | 1.0-1.2 ml/min | ||||||||||||||||||||
| Column temperature | ambient (~25 °C) | ||||||||||||||||||||
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. | ||||||||||||||||||||
| Method of Analysis | HPLC | ||||||||||||||||||||
| Parameter | 3-Methoxytyramine, Metanephrine, Normetanephrine | ||||||||||||||||||||
| Complete Specification |
HPLC parametersFor the Chromsystems HPLC analysis of metanephrines in urine any isocratic HPLC system with electrochemical detector is suitable.
|
||||||||||||||||||||
| Additional Info | HPLC parametersFor the Chromsystems HPLC analysis of metanephrines in urine any isocratic HPLC system with electrochemical detector is suitable. |
||||||||||||||||||||
| Potential | aproxx. +780 to +840 mV |
