Parameter Set Antimycotic Drugs/EXTENDED - LC-MS/MS

Order No.: 92922/XT/RUO
Parameters:
5-Flucytosine, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole

Encompasses 11 analytes
3PLUS1® Multilevel Calibrator Set and MassCheck® controls
Part of the modular system MassTox® Series A

5-Flucytosine

Anidulafungin

Caspofungin

Fluconazole

Isavuconazole

Itraconazole

Hydroxyitraconazole

Ketoconazole

Micafungin

Posaconazole

Voriconazole

Clinical relevance

This parameter set allows the quantitative determination of anidulafungin, caspofungin, fluconazole, 5-flucytosine, isavuconazole, itraconazole, hydroxyitraconazole, ketoconazole, micafungin, posaconazole and voriconazole in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual.
Specimen Serum/Plasma
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.
Run Time 3.2 min
Injection Volume 0.5 to 50 µl (mass spectrometer dependent)
Flow Rate 0.6 ml/min
Column Temperature +20 to +25 °C
Gradient binary
Ionisation ESI positive and negative, polarity switch possible
MS/MS Mode MRM
Parameters 5-Flucytosine, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole

92922/XT MassTox® Parameter Set Antimycotic Drugs/EXTENDED

Order No. MassTox® Basic Kit A
92111/200/RUO Basic Kit A for 200 tests
92111/1000/RUO Basic Kit A for 1000 tests

 

Order No. MassTox® Basic Kit A (Quantities in kit for 200 tests)
92001/RUO Mobile Phase 1, 1000 ml (1x)
92002/RUO Mobile Phase 2, 1000 ml (1x)
92003/RUO Precipitation Reagent, 50 ml (1x)
92005/RUO Extraction Buffer, 5 ml (1x)
92007/RUO Dilution Buffer 1, 50 ml (1x)
92008/RUO Dilution Buffer 2, 50 ml (1x)
92009/RUO Rinsing Solution, 1000 ml (1x)
33006 Reaction Vials, transparent, 1.5 ml, 100 pcs. (2x)

 

92922/XT MassTox® Parameter Set Antimycotic Drugs/EXTENDED components
92051/XT 3PLUS1® Multilevel Plasma Calibrator Set Antimycotic Drugs/EXTENDED (lyoph.), 4 x 1 ml
0253/XT MassCheck® Antimycotic Drugs/EXTENDED Plasma Control, Level I (lyoph.), 5 x 1 ml
0254/XT MassCheck® Antimycotic Drugs/EXTENDED Plasma Control, Level II (lyoph.), 5 x 1 ml
92644/XT/RUO Internal Standard Mix Antimycotic Drugs/EXTENDED, 1 x 3.8 ml (for 200 tests)

 

Order No. Required components (not included in the kit or the parameter set)
92110 MassTox® TDM MasterColumn® A, equilibrated, with test chromatogram, 1 pc.
92039/XT/RUO Tuning Mix Antimycotic Drugs/EXTENDED (Analytes and Internal Standards), 1 ml

 

Order No. General lab equipment (non-CE/IVD products)
15010 PEEK Prefilter Housing, 1 pc.
15011 PEEK-encased Prefilter, 2 µm, 5 pcs.
15070 Stainless Steel Prefilter Housing, 1 pc.
15071 Stainless Steel Prefilter, 0.5 μm, 5 pcs.
33006 Reaction Vials, transparent, 1.5 ml, 100 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

5-Flucytosine

Anidulafungin

Caspofungin

Fluconazole

Isavuconazole

Itraconazole

Hydroxyitraconazole

Ketoconazole

Micafungin

Posaconazole

Voriconazole

Clinical relevance

This parameter set allows the quantitative determination of anidulafungin, caspofungin, fluconazole, 5-flucytosine, isavuconazole, itraconazole, hydroxyitraconazole, ketoconazole, micafungin, posaconazole and voriconazole in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual.
Specimen Serum/Plasma
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.
Run Time 3.2 min
Injection Volume 0.5 to 50 µl (mass spectrometer dependent)
Flow Rate 0.6 ml/min
Column Temperature +20 to +25 °C
Gradient binary
Ionisation ESI positive and negative, polarity switch possible
MS/MS Mode MRM
Parameters 5-Flucytosine, Anidulafungin, Caspofungin, Fluconazole, Hydroxyitraconazole, Isavuconazole, Itraconazole, Ketoconazole, Micafungin, Posaconazole, Voriconazole

92922/XT MassTox® Parameter Set Antimycotic Drugs/EXTENDED

Order No. MassTox® Basic Kit A
92111/200/RUO Basic Kit A for 200 tests
92111/1000/RUO Basic Kit A for 1000 tests

 

Order No. MassTox® Basic Kit A (Quantities in kit for 200 tests)
92001/RUO Mobile Phase 1, 1000 ml (1x)
92002/RUO Mobile Phase 2, 1000 ml (1x)
92003/RUO Precipitation Reagent, 50 ml (1x)
92005/RUO Extraction Buffer, 5 ml (1x)
92007/RUO Dilution Buffer 1, 50 ml (1x)
92008/RUO Dilution Buffer 2, 50 ml (1x)
92009/RUO Rinsing Solution, 1000 ml (1x)
33006 Reaction Vials, transparent, 1.5 ml, 100 pcs. (2x)

 

92922/XT MassTox® Parameter Set Antimycotic Drugs/EXTENDED components
92051/XT 3PLUS1® Multilevel Plasma Calibrator Set Antimycotic Drugs/EXTENDED (lyoph.), 4 x 1 ml
0253/XT MassCheck® Antimycotic Drugs/EXTENDED Plasma Control, Level I (lyoph.), 5 x 1 ml
0254/XT MassCheck® Antimycotic Drugs/EXTENDED Plasma Control, Level II (lyoph.), 5 x 1 ml
92644/XT/RUO Internal Standard Mix Antimycotic Drugs/EXTENDED, 1 x 3.8 ml (for 200 tests)

 

Order No. Required components (not included in the kit or the parameter set)
92110 MassTox® TDM MasterColumn® A, equilibrated, with test chromatogram, 1 pc.
92039/XT/RUO Tuning Mix Antimycotic Drugs/EXTENDED (Analytes and Internal Standards), 1 ml

 

Order No. General lab equipment (non-CE/IVD products)
15010 PEEK Prefilter Housing, 1 pc.
15011 PEEK-encased Prefilter, 2 µm, 5 pcs.
15070 Stainless Steel Prefilter Housing, 1 pc.
15071 Stainless Steel Prefilter, 0.5 μm, 5 pcs.
33006 Reaction Vials, transparent, 1.5 ml, 100 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: