MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate
6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
Chromatographic separation of analytes
Isotopically labelled internal standard for each analyte
Assay validated according to IVDR (=> Declaration of Conformity)
3-Methoxytyramine
Metanephrine
Normetanephrine
MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plates
For 2 x 96 tests
Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).
Clinical relevance
This assay allows the quantitative determination of free metanephrine, free normetanephrine and free 3-methoxytyramine in human plasma samples by LC-MS/MS. The assay can be employed as an aid to diagnose and monitor suspected pheochromocytoma or paraganglioma. Furthermore, the assay is an aid to diagnosing and monitoring suspected neuroblastoma.
Product advantages
• 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
• Chromatographic separation of analytes
• Isotopically labelled internal standard for each analyte
Assay characteristics
Sample clean-up is performed by efficient solid phase extraction either in 96 SPE well plates or SPE columns (Sample Clean Up Columns). Solid phase extraction does not require equilibration or conditioning steps and accomplishes analyte enrichment without a vaporisation step. Each analyte is measured in relation to a specific isotope-labelled internal standard to ensure that reproducible and reliable quantitative results are obtained. The 6PLUS1® Multilevel Calibrator Set and MassCheck® controls are obtained from human plasma to minimise matrix-induced differences.
Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 2 x 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Metanephrine: 2.8 ng/l – 5650 ng/l Different systems might show different performance data. |
| Specimen | Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 5 min |
| Injection Volume | 5 to 50 µl (mass spectrometer dependent) |
| Flow Rate | 0.5–2.0 ml/min |
| Column Temperature | + 25 °C |
| Gradient | Binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 3-Methoxytyramine, Metanephrine, Normetanephrine |
81000 MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plates
For 2 x 96 tests
| Order No. | Kit components (Quantities in kit) |
| 81001 | Mobile Phase A, 750 ml (1x) |
| 81002 | Mobile Phase B, 450 ml (1x ) |
| 81003 | Dilution Buffer, 50 ml (2x) |
| 81004 | Internal Standard Mix, 2 x 2,5 ml (1x) |
| 81005 | Wash Buffer 1, 90 ml (2x) |
| 81006 | Wash Buffer 2, 90 ml (4x) |
| 81007 | Elution Buffer, 25 ml (2x) |
| 81009 | Rinsing Solution, 500 ml (1x) |
| 81057 | 96 SPE Well Plates, 1 pc. (2x) |
| 81058 | Collection Plates, 3 pcs. (1x) |
| 81059 | Pierceable Adhesive Seals for 96 well plates, 3 pcs. (1x) |
| Order No. | Required components (not included in the kit) |
| 81100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 81056 | Waste Plates, 3 pcs. |
| 81039 | 6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines, 7 x 3.0 ml |
| 0384 | MassCheck® Free Metanephrines Plasma Control, Level I, 5 x 3.0 ml |
| 0385 | MassCheck® Free Metanephrines Plasma Control, Level II, 5 x 3.0 ml |
| 0386 | MassCheck® Free Metanephrines Plasma Control, Level III, 5 x 3.0 ml |
| 81015 | Tuning Mix, Analytes and Internal Standards, for first installation and optimisation, 1 x 1 ml |
| Order No. | Lab equipment |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 0.5 μm, 5 pcs. |
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 81059MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
Analytical Column Free Metanephrines in Plasma - LC-MS/MSOrder no.: 81100MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Free MetanephrinesOrder no.: 81039MassChrom® Free Metanephrines in Plasma – LC-MS/MS -
MassCheck® Free Metanephrines Plasma ControlsOrder no.: 0384/0385/0386MassChrom® Free Metanephrines in Plasma – LC-MS/MS -
Tuning Mix Free Metanephrines in PlasmaOrder no.: 81015MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Free MetanephrinesOrder no.: 81039MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
MassCheck® Free Metanephrines Plasma ControlsOrder no.: 0384/0385/0386MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
Analytical Column Free Metanephrines in Plasma - LC-MS/MSOrder no.: 81100
MassChrom® Free Metanephrines in Plasma - LC-MS/MS
3-Methoxytyramine
Metanephrine
Normetanephrine
MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plates
For 2 x 96 tests
Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).
Clinical relevance
This assay allows the quantitative determination of free metanephrine, free normetanephrine and free 3-methoxytyramine in human plasma samples by LC-MS/MS. The assay can be employed as an aid to diagnose and monitor suspected pheochromocytoma or paraganglioma. Furthermore, the assay is an aid to diagnosing and monitoring suspected neuroblastoma.
Product advantages
• 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
• Chromatographic separation of analytes
• Isotopically labelled internal standard for each analyte
Assay characteristics
Sample clean-up is performed by efficient solid phase extraction either in 96 SPE well plates or SPE columns (Sample Clean Up Columns). Solid phase extraction does not require equilibration or conditioning steps and accomplishes analyte enrichment without a vaporisation step. Each analyte is measured in relation to a specific isotope-labelled internal standard to ensure that reproducible and reliable quantitative results are obtained. The 6PLUS1® Multilevel Calibrator Set and MassCheck® controls are obtained from human plasma to minimise matrix-induced differences.
Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 2 x 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Metanephrine: 2.8 ng/l – 5650 ng/l Different systems might show different performance data. |
| Specimen | Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 5 min |
| Injection Volume | 5 to 50 µl (mass spectrometer dependent) |
| Flow Rate | 0.5–2.0 ml/min |
| Column Temperature | + 25 °C |
| Gradient | Binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 3-Methoxytyramine, Metanephrine, Normetanephrine |
81000 MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plates
For 2 x 96 tests
| Order No. | Kit components (Quantities in kit) |
| 81001 | Mobile Phase A, 750 ml (1x) |
| 81002 | Mobile Phase B, 450 ml (1x ) |
| 81003 | Dilution Buffer, 50 ml (2x) |
| 81004 | Internal Standard Mix, 2 x 2,5 ml (1x) |
| 81005 | Wash Buffer 1, 90 ml (2x) |
| 81006 | Wash Buffer 2, 90 ml (4x) |
| 81007 | Elution Buffer, 25 ml (2x) |
| 81009 | Rinsing Solution, 500 ml (1x) |
| 81057 | 96 SPE Well Plates, 1 pc. (2x) |
| 81058 | Collection Plates, 3 pcs. (1x) |
| 81059 | Pierceable Adhesive Seals for 96 well plates, 3 pcs. (1x) |
| Order No. | Required components (not included in the kit) |
| 81100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 81056 | Waste Plates, 3 pcs. |
| 81039 | 6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines, 7 x 3.0 ml |
| 0384 | MassCheck® Free Metanephrines Plasma Control, Level I, 5 x 3.0 ml |
| 0385 | MassCheck® Free Metanephrines Plasma Control, Level II, 5 x 3.0 ml |
| 0386 | MassCheck® Free Metanephrines Plasma Control, Level III, 5 x 3.0 ml |
| 81015 | Tuning Mix, Analytes and Internal Standards, for first installation and optimisation, 1 x 1 ml |
| Order No. | Lab equipment |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 0.5 μm, 5 pcs. |
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 81059
MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
Analytical Column Free Metanephrines in Plasma - LC-MS/MSOrder no.: 81100
MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Free MetanephrinesOrder no.: 81039MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
MassCheck® Free Metanephrines Plasma ControlsOrder no.: 0384/0385/0386MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
Tuning Mix Free Metanephrines in PlasmaOrder no.: 81015
MassChrom® Free Metanephrines in Plasma - LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Free MetanephrinesOrder no.: 81039MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
MassCheck® Free Metanephrines Plasma ControlsOrder no.: 0384/0385/0386MassChrom® Free Metanephrines in Plasma – LC-MS/MS
-
Analytical Column Free Metanephrines in Plasma - LC-MS/MSOrder no.: 81100
MassChrom® Free Metanephrines in Plasma - LC-MS/MS