Catecholamines in Urine, Combined analysis - HPLC
Short analysis time < 6 min
Only 1 HPLC column and 1 Mobile Phase for 2020/COMBI and 6000/COMBI
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
3-Methoxytyramine
Metanephrine
Normetanephrine
Clinical relevance
The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.
Product advantages
- Short analysis time of less than 6 minutes
- No changes to setup are needed due to identical column and mobile phase
This assay enables the combined determination of catecholamines together with metanephrines in urine (2020/COMBI). It is an exceptionally sensitive and reliable method for detecting pheochromocytomas with an analysis time of less than 6 minutes. The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 1 μg/l |
| Upper Limit of Quantification | 1–1000 μg/l |
| Intraassay | CV = 0.9-2.7 % |
| Interassay | CV = 2.1-4.1 % |
| Recovery | 81-88 % |
| Specimen | Urine |
| Sample Preparation | Stabilisation
Extraction
Elution
HPLC Analysis
|
| Run Time | < 6 min |
| Injection Volume | 10 µl |
| Flow Rate | 0.9 ml/min |
| Column Temperature | 30 °C |
| Potential | +580/+640 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline (Norepinephrine), Normetanephrine |
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Mobile PhaseOrder no.: 2031/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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Stabilisation ReagentOrder no.: 6010/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
Adrenaline (Epinephrine)
Dopamine
Noradrenaline (Norepinephrine)
3-Methoxytyramine
Metanephrine
Normetanephrine
Clinical relevance
The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.
Product advantages
- Short analysis time of less than 6 minutes
- No changes to setup are needed due to identical column and mobile phase
This assay enables the combined determination of catecholamines together with metanephrines in urine (2020/COMBI). It is an exceptionally sensitive and reliable method for detecting pheochromocytomas with an analysis time of less than 6 minutes. The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 1 μg/l |
| Upper Limit of Quantification | 1–1000 μg/l |
| Intraassay | CV = 0.9-2.7 % |
| Interassay | CV = 2.1-4.1 % |
| Recovery | 81-88 % |
| Specimen | Urine |
| Sample Preparation | Stabilisation
Extraction
Elution
HPLC Analysis
|
| Run Time | < 6 min |
| Injection Volume | 10 µl |
| Flow Rate | 0.9 ml/min |
| Column Temperature | 30 °C |
| Potential | +580/+640 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline (Norepinephrine), Normetanephrine |
-
Mobile PhaseOrder no.: 2031/COMBI
Combined analysis of catecholamines/metanephrines in urine - HPLC
-
Stabilisation ReagentOrder no.: 6010/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
-
HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
-
HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC