Vitamin B2 in Whole Blood - HPLC
Safe results by stabilisation of FAD
Simultaneous determination of FMN
Highly stable calibrators and controls
Flavin adenine dinucleotide (FAD)
Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)
The present method allows the rapid and simple determination of riboflavin, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) in whole blood using an isocratic HPLC system. Due to their native fluorescence, the analytes can be detected directly, without derivatisation. Sample preparation is restricted to one effective protein precipitation step immediately after addition of the extraction buffer and subsequent stabilisation of the analytes. A dedicated HPLC column and a mobile phase, optimised for the specific separation, permit high performance in HPLC quantification.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | approx. 9 min |
| Injection Volume | 50 µl |
| Flow Rate | 1.2 ml/min |
| Column Temperature | ambient (~20 °C) |
| Wavelengths | EX 465 nm EM 525 nm |
| Additional Info | For the analysis of vitamin B2 in whole blood every isocratic system with fluorescence detector is suitable. |
| Parameters | Flavin adenine dinucleotide (FAD), Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin) |
Flavin adenine dinucleotide (FAD)
Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)
The present method allows the rapid and simple determination of riboflavin, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) in whole blood using an isocratic HPLC system. Due to their native fluorescence, the analytes can be detected directly, without derivatisation. Sample preparation is restricted to one effective protein precipitation step immediately after addition of the extraction buffer and subsequent stabilisation of the analytes. A dedicated HPLC column and a mobile phase, optimised for the specific separation, permit high performance in HPLC quantification.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | approx. 9 min |
| Injection Volume | 50 µl |
| Flow Rate | 1.2 ml/min |
| Column Temperature | ambient (~20 °C) |
| Wavelengths | EX 465 nm EM 525 nm |
| Additional Info | For the analysis of vitamin B2 in whole blood every isocratic system with fluorescence detector is suitable. |
| Parameters | Flavin adenine dinucleotide (FAD), Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin) |