Serotonin in Urine - HPLC

Order No.: 4000, for 100 tests
Parameters:
Serotonin

Now easier pH adjustment with pH indicator
Easy sample preparation
Short analysis time


Serotonin

Clinical relevance

Serotonin (5-hydroxytryptamine) is a biogenic amine. In humans, serotonin is involved in a large number of physiological processes, e.g. it acts as a tissue hormone and neurotransmitter in the central nervous system, the enteric nervous system and the cardiovascular system. A large amount of serotonin is found in the chromaffin cells of the gastrointestinal tract where it is part of the gastrointestinal hormone system. When released into the capillary blood, serotonin is almost absorbed by the thrombocytes.

A number of diseases are associated with pathological changes in the serotonin metabolism. For the routine, the diagnosis of carcinoid tumours together with the analysis of 5-hydroxyindoleacetic acid (5-HIAA) is very important.

 

Product advantages

  • Simple and efficient sample preparation
  • Fast results due to short analysis times
  • Long column life times

 

This kit allows for reliable HPLC determination of serotonin in urine as part of routine diagnostic laboratory testing. Easy and fast sample preparation leads to exact and reproducible results. The chromatographic determination requires an isocratic HPLC system with electrochemical detection. Calibrator and quality controls are available separately – they are produced from pure human matrices to prevent matrix-associated measurement variabilities.

Alternatively, Chromsystems offers a kit for determining serotonin in serum/plasma and whole blood (# 3030).

  

Kit content

Mobile Phase, 1000 ml
Serotonin Calibration Standard, 10 ml
Internal Standard, 10 ml
Neutralisation Buffer, 300 ml
Wash Buffer, 300 ml
Elution Buffer, 500 ml
Sample Clean Up Columns, 100 pcs.

More Information
Number of Tests 100
Limit of quantification 5 µg/l
Linearity up to 1000 µg/l
Recovery ~ 99 %
Intraassay CV < 2 %
Interassay CV < 3 %
Analysis Time aproxx. 8 min
Sample Preparation
  • Mix 4 ml urine with 100 µl Internal Standard and 6 ml Neutralisation Buffer.
  • Add 2 N NaOH until the colour changes from yellow to green or green-grey (see figure).

 

 

  • Resuspend Sample Clean Up Column and allow buffer to flow out.
  • Apply entire urine dilution to the column, discard effluent.
  • Rinse the column with 1 volume of water, discard effluent.
  • Apply 3 ml Wash Buffer, discard effluent.
  • Apply 20 ml Elution Buffer, collect eluate, mix well.
  • Inject 20 to 50 µl eluate into the HPLC system.
Sample Stability At +2 to +8 °C at least 5 days. Aliquot and freeze for longer storage.
Specimen 24 h urine (containing 10 ml 25 % HCl) or spontaneous urine (refer data to urine creatinine).
Pre-analytic Treatment

Note:

The following food and drugs can falsify the results of the determination: pineapples, eggplants, avocados, bananas, redcurrants, kiwis, melons, mirabelles, gooseberries, tomatoes, walnuts, plums, methocarbamol, mephenesin, guaifenesin, paracetamol, salicylic acid.

Injection Volume 20-50 µl
Flow rate 0.8-1.3 ml/min
Column temperature ambient (~25 °C)
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Serotonin
Complete Specification
Linearity: up to 1000 µg/l
Limit of quantification: 5 µg/l
Intra-assay: CV < 2 %
Inter-assay: CV < 3 %
Recovery: ~ 99 %
Run time: aproxx. 8 min

HPLC parameters

For the HPLC analysis of serotonin in urine any isocratic HPLC system with electrochemical detector is suitable.

Injection volume: 20-50 µl
Flow rate: 0.8-1.3 ml/min
Potential: aproxx. +400 to +500 mV
Column temperature: ambient (~25 °C)
Additional Info

For the HPLC analysis of serotonin in urine any isocratic HPLC system with electrochemical detector is suitable.

Potential approx. +400 to +500 mV
  1. 3031 HPLC mobile phase serotonin
    Mobile Phase
    Order no.: 3031
    Serotonin in Serum/Plasma/Whole Blood or in Urine - HPLC
    Details
  2. 3031 HPLC mobile phase serotonin
    Mobile Phase, 10 x 1000 ml
    Order no.: 3032
    Serotonin in Serum/Plasma/Whole Blood or in Urine - HPLC
    Details
  3. 3003 HPLC serotonin calibration standard
    Serotonin Calibration Standard
    Order no.: 3003
    For Serotonin in Urine - HPLC
    Details
  4. 4004 HPLC internal standard serotonin urine
    Internal Standard
    Order no.: 4004
    Serotonin in Urine - HPLC
    Details
  5. 4055 HPLC neutralisation buffer serotonin urine
    Neutralisation Buffer
    Order no.: 4055
    Serotonin in Urine - HPLC
    Details
  6. 4006 HPLC wash buffer serotonin urine
    Wash Buffer
    Order no.: 4006
    Serotonin in Urine - HPLC
    Details
  7. 4007 HPLC elution buffer serotonin urine
    Elution Buffer
    Order no.: 4007
    Serotonin in Urine - HPLC
    Details
  8. 4008 HPLC sample clean up columns serotonin
    Sample Clean Up Columns
    Order no.: 4008
    Serotonin in Urine - HPLC
    Details
  1. 4009 HPLC urine calibration standard serotonin
    Urine Calibration Standard
    Order no.: 4009
    Serotonin in Urine - HPLC
    Details
  2. 3003 HPLC serotonin calibration standard
    Serotonin Calibration Standard
    Order no.: 3003
    For Serotonin in Urine - HPLC
    Details
  1. 0040 0050 HPLC endocrine urine controls
    Endocrine Urine Controls
    Order no.: 0040/0050
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. 4100 HPLC column serotonin urine
    HPLC column
    Order no.: 4100
    Serotonin in Urine
    Details
  3. PEEK-encased Prefilter, 5 µm
    Details
As a customer please login or register to gain full access.