Sample Prep Set Total Metanephrines (free + conjugated) with Sample Clean Up Columns

Order No.: 80700/C, for 96 tests
Parameters:
3-Methoxytyramine, Metanephrine, Normetanephrine

Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With Sample Clean Up Columns, for manual sample prep procedure

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of total metanephrine, normetanephrine and 3-methoxytyramine in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of catecholamine-secreting tumours.

 

Product advantages

  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

There is also a method using 96 SPE well plates (80700/96) as well as the corresponding fully automated workflow solution available for laboratories with a higher sample throughput.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Linearity

Metanephrine, Normetanephrine up to 20 mg/l
3-Methoxytyramine up to 10 mg/l

Recovery 92–107 %
Intraassay CV = 2.5–5.6 %
Interassay CV = 3.2–4.9 %
Analysis Time 4 min
Specimen 24h urine or spontaneous urine
Pre-analytic Treatment 24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl.
Sample Preparation
  • Pipette 200 μl sample/calibrator/MassCheck® control into a reaction vial.
  • Add 50 μl Internal Standard Mix+ 25 μl Hydrolysis Buffer.
  • Close reaction vial, mix briefly.
  • Hydrolyse for 30 min at 95 °C.
  • Cool for at least 10 min at +2 to +8 °C.
  • Pipette 50 μl of the hydrolysate into a Sample Clean Up Column.
  • Add 600 μl Neutralisation Buffer.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Change collection vials. (Use glass vials, see instruction manual).
  • Pipette 500 μl Elution Buffer into each Sample Clean Up Column and centrifuge 2 min at 100 x g.
  • Pipette 500 μl Dilution Buffer into each glass vial.
  • Close glass vials.
  • Vortex, transfer to autosampler.
  • Inject up to 20 μl into the LC-MS/MS system.
Sample Stability Samples are stable for at least 7 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.
Injection Volume ≤ 20 µl
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter 3-Methoxytyramine, Metanephrine, Normetanephrine
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