Parameter Set Antiepileptic Drugs - LC-MS/MS

Order No.: 92921/RUO

Parameters:

10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Chromatogram

Parameters

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Oxcarbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophyllie

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

Description

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antiepileptic Drugs allows for the fast quantitative determination of 26 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

Technical Data

More Information
Analysis Time	2.5 – 3.5 min
Sample Preparation	Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.
Sample Stability	Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen	Serum/Plasma
Injection Volume	0.2 – 50 µl
Gradient	Starting point: 0% Mobile Phase 2 Group 1 0.00→2.00 min, 100 % Mobile Phase 2 2.00-2.60 min, 100 % Mobile Phase 2 2.61-3.50 min, 0 % Mobile Phase 2 Group 2 and 3 0.00→1.00 min, 100 % Mobile Phase 2 1.00-1.60 min, 100 % Mobile Phase 2 1.61-2.50 min, 0 % Mobile Phase 2 Group 4 and 5 0.00→1.00 min, 100 % Mobile Phase 2 1.00-1.80 min, 100 % Mobile Phase 2 1.81-2.70 min, 0 % Mobile Phase 2
Ionisation	ESI positive and negative
MS/MS-Mode	MRM
Additional Info	We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note	The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis	LC-MS/MS
Parameter	10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Components

3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDED
Order no.: 92025/XT

Details
MassCheck® Antiepileptic Drugs/EXTENDED Plasma Controls
Order no.: 0249/XT;0250/XT;0251/XT

Details
Internal Standard Mix Antiepileptic Drugs
Order no.: 92546/RUO

Component of the Parameter Set Antiepileptic Drugs, available separately

Details

Calibrators / Controls

3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDED
Order no.: 92025/XT

Details

MassCheck® Antiepileptic Drugs/EXTENDED Plasma Controls
Order no.: 0249/XT;0250/XT;0251/XT

Details

Accessories

MasterColumn® A - LC-MS/MS
Order no.: 92110

Analytical column for MassTox® Series A

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 1
Order no.: 92034/XT/RUO

Tuning Mix 1 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 2
Order no.: 92035/XT/RUO

Tuning Mix 2 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 3
Order no.: 92036/XT/RUO

Tuning Mix 3 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 4
Order no.: 92037/XT/RUO

Tuning Mix 4 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 5
Order no.: 92038/XT/RUO

Tuning Mix 5 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
PEEK Prefilter Housing
Order no.: 15010

Details
PEEK-encased Prefilter, 2 µm
Order no.: 15011

Details