Parameter Set Antiepileptic Drugs - LC-MS/MS

Order No.: 92921/RUO
Parameters:
10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Oxcarbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophyllie

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antiepileptic Drugs allows for the fast quantitative determination of 26 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.



More Information
Analysis Time 2.5 – 3.5 min
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.
Sample Stability Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Ionisation ESI positive and negative
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
  1. 0249XT 0250XT 0251XT LCMS TDM Series A controls antiepileptic drugs
    Details
  2. Internal Standard Mix Antiepileptic Drugs
    Internal Standard Mix Antiepileptic Drugs
    Order no.: 92546/RUO
    Component of the Parameter Set Antiepileptic Drugs, available separately
    Details
  1. 0249XT 0250XT 0251XT LCMS TDM Series A controls antiepileptic drugs
    Details
  1. 92110 TDM Series A Master Column
    MasterColumn® A - LC-MS/MS
    Order no.: 92110

    Analytical column for MassTox® Series A

    Details
  2. Tuning Mix Antiepileptic Drugs/EXTENDED 1
    Tuning Mix Antiepileptic Drugs/EXTENDED 1
    Order no.: 92034/XT/RUO
    Tuning Mix 1 for the Parameter Set Antiepileptic Drugs - LC-MS/MS
    Details
  3. Tuning Mix Antiepileptic Drugs/EXTENDED 2
    Tuning Mix Antiepileptic Drugs/EXTENDED 2
    Order no.: 92035/XT/RUO
    Tuning Mix 2 for the Parameter Set Antiepileptic Drugs - LC-MS/MS
    Details
  4. Tuning Mix Antiepileptic Drugs/EXTENDED 3
    Tuning Mix Antiepileptic Drugs/EXTENDED 3
    Order no.: 92036/XT/RUO
    Tuning Mix 3 for the Parameter Set Antiepileptic Drugs - LC-MS/MS
    Details
  5. Tuning Mix Antiepileptic Drugs/EXTENDED 4
    Tuning Mix Antiepileptic Drugs/EXTENDED 4
    Order no.: 92037/XT/RUO
    Tuning Mix 4 for the Parameter Set Antiepileptic Drugs - LC-MS/MS
    Details
  6. Tuning Mix Antiepileptic Drugs/EXTENDED 5
    Tuning Mix Antiepileptic Drugs/EXTENDED 5
    Order no.: 92038/XT/RUO
    Tuning Mix 5 for the Parameter Set Antiepileptic Drugs - LC-MS/MS
    Details
  7. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  8. PEEK-encased Prefilter, 2 µm
    Details