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MassChrom® Amino Acid Analysis in Plasma/Serum with Reaction Vials

Order No.: 75111/RUO, for 3 x 96 tests
Parameters:
Amino Acids

Quantitative analysis of 48 amino acids
No derivatisation
Short run times
Excellent separation of isobaric analytes
Each analyte safeguarded by its own internal standard

main product photo
Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine		 Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine		 Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Leucine
Isoleucine
Allo-Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Phenylalanine		 Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

The 7 analytes highlighted in red can be measured with both the full panel and the specific panel.

This assay is designed for the quantitative determination of amino acids in plasma/serum by LC-MS/MS. The method covers 48 amino acids and derivatives that are analysed within 20 min in one single run. Alternatively, the same assay allows the determination of 7 specific analytes in a much shorter run time (8-9 min) using a modified gradient. Both panels use the identical chromatographic set up, research sample preparation procedure, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls. In addition, each of the 48 analytes is safeguarded by an own internal standard. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.

More Information
Number of Tests 3 x 96
Sample Preparation
  • Pipette 50 μl reconstituted Internal Standard Mix
    + 25 μl research sample/calibrator/MassCheck® control
    + 400 μl Precipitation Reagent into a reaction vial.
  • Mix for 30 s (vortex) and centrifuge for 5 min at 16000 x g.
  • Transfer supernatant into autosampler vial.
  • Inject 2–5 μl into the LC-MS/MS system.
Sample Stability Stability of research samples depends on the specific analyte. Further information can be obtained from the instruction manual.
Specimen Plasma/Serum
Injection Volume 2–5 μl
Column temperature ambient (~ 25 °C)
Gradient

Full panel: binary gradient, flow gradient

Specific panel: isocratic
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Amino Acids
  1. J0601 Autosampler Vials - amber glass
    Autosampler Vials, screw neck, amber glass, 1.5 ml
    Order no.: J0601
    Details
  2. PP Screw-on caps, rubber/PTFE septa, 9 mm
    PP Screw-on caps, rubber/PTFE septa, 9 mm
    Order no.: J0504
    Details
  3. Mobile Phase A
    Mobile Phase A
    Order no.: 75001/RUO

    MasChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  4. Mobile Phase B
    Mobile Phase B
    Order no.: 75002/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  5. Rinsing Solution
    Rinsing Solution
    Order no.: 75009/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  6. Internal Standard Set Amino Acid Analysis
    Internal Standard Set Amino Acid Analysis
    Order no.: 75146/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  7. Precipitation Reagent
    Precipitation Reagent
    Order no.: 75105/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  8. Reaction vials, transparent
    Reaction vials, transparent
    Order no.: 33006/RUO
     
    Details
  1. 75128 3PLUS1® Multilevel Plasma Calibrator Set Amino Acid Analysis
    3PLUS1® Multilevel Plasma Calibrator Set Amino Acid Analysis
    Order no.: 75128
     
    Details
  1. Antibiotics Plasma Controls 0182 0183 0184
    MassCheck® Amino Acid Analysis Plasma Controls
    Order no.: 0471/0472/0473

    Order no.: 0471/0472/0473

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. J0601 Autosampler Vials - amber glass
    Autosampler Vials, screw neck, amber glass, 1.5 ml
    Order no.: J0601
    Details
  3. J0410 J0602
    PP Screw-on caps, pierceable silicone/PTFE septa, 1.0 mm
    Order no.: J0410
    Details
  4. PP Screw-on caps, rubber/PTFE septa, 9 mm
    PP Screw-on caps, rubber/PTFE septa, 9 mm
    Order no.: J0504
    Details
  5. Micro-inserts for autosampler vials
    Micro-inserts for Autosampler Vials, clear glass
    Order no.: J0505
    Details
  6. System Check Solution
    System Check Solution
    Order no.: 75010/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  7. Tuning Mix 1 Amino Acid Analysis
    Tuning Mix 1 Amino Acid Analysis
    Order no.: 75015/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  8. Tuning Mix 2 Amino Acid Analysis
    Tuning Mix 2 Amino Acid Analysis
    Order no.: 75016/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  9. Tuning Mix 3 Amino Acid Analysis
    Tuning Mix 3 Amino Acid Analysis
    Order no.: 75017/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  10. Tuning Mix 4 Amino Acid Analysis
    Tuning Mix 4 Amino Acid Analysis
    Order no.: 75018/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  11. Tuning Mix 5 Amino Acid Analysis
    Tuning Mix 5 Amino Acid Analysis
    Order no.: 75019/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details