Serotonin in Serum/Plasma/Whole Blood - HPLC

Order No.: 3030, for 100 tests
Parameters:
Serotonin

Easy sample preparation
Short run time

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Serotonin

Clinical relevance

Serotonin (5-hydroxytryptamine) is a biogenic amine. In humans, serotonin is involved in a large number of physiological processes, e.g. it acts as a tissue hormone and neurotransmitter in the central nervous system, the enteric nervous system and the cardiovascular system. A large amount of serotonin is found in the chromaffin cells of the gastrointestinal tract where it is part of the gastrointestinal hormone system. When released into the capillary blood, serotonin is almost absorbed by the thrombocytes.

A number of diseases are associated with pathological changes in the serotonin metabolism. For the routine, the diagnosis of carcinoid tumours together with the analysis of 5-hydroxyindoleacetic acid (5-HIAA) is very important.

This assay allows the quantitative determination of serotonin in serum/plasma and whole blood with HPLC (high performance liquid chromatography) with electrochemical detection.
It is intended as a monitoring test for patients with suspected serotonin-secreting tumours.

 

Product advantages

• Easy sample preparation
• Short Run time

 

Assay characteristics

The chromatographic determination requires an isocratic HPLC system with electrochemical detection. Calibrator and quality controls are available separately – they are produced from human matrices to prevent matrix-associated measurement variabilities.

Alternatively, Chromsystems offers an assay for determining serotonin in urine (order no. 4000).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation 3 µg/l – 1000 µg/l
Specimen Serum/Plasma/Whole Blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

Serum specimens

  • Vortex 100 µl serum + 100 µl Internal Standard + 100 µl Precipitation Reagent in a reaction vial for 30 s.
  • Incubate 10 min at +2 to +8 °C.
  • Centrifuge 10 min at approx. 16 000 rpm.
  • Inject 20 µl supernatant into the HPLC system.

Plasma specimens

  • For the determination of serotonin in plasma it is necessary to dilute the Internal Standard 1:10 with ultrapure water (HPLC grade).

Whole blood specimens

  • For the step by step description of the sample preparation of whole blood specimens, please refer to the instruction manual.
Run Time < 8 min
Injection Volume 20 µl
Flow Rate 1.0 to 1.2 ml/min
Column Temperature ambient (~25 °C)
Potential approx. 400 to 500 mV
Additional Info

For the HPLC analysis of serotonin any isocratic HPLC system with electrochemical detector is suitable.

Parameters Serotonin
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Serotonin

Clinical relevance

Serotonin (5-hydroxytryptamine) is a biogenic amine. In humans, serotonin is involved in a large number of physiological processes, e.g. it acts as a tissue hormone and neurotransmitter in the central nervous system, the enteric nervous system and the cardiovascular system. A large amount of serotonin is found in the chromaffin cells of the gastrointestinal tract where it is part of the gastrointestinal hormone system. When released into the capillary blood, serotonin is almost absorbed by the thrombocytes.

A number of diseases are associated with pathological changes in the serotonin metabolism. For the routine, the diagnosis of carcinoid tumours together with the analysis of 5-hydroxyindoleacetic acid (5-HIAA) is very important.

This assay allows the quantitative determination of serotonin in serum/plasma and whole blood with HPLC (high performance liquid chromatography) with electrochemical detection.
It is intended as a monitoring test for patients with suspected serotonin-secreting tumours.

 

Product advantages

• Easy sample preparation
• Short Run time

 

Assay characteristics

The chromatographic determination requires an isocratic HPLC system with electrochemical detection. Calibrator and quality controls are available separately – they are produced from human matrices to prevent matrix-associated measurement variabilities.

Alternatively, Chromsystems offers an assay for determining serotonin in urine (order no. 4000).

Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation 3 µg/l – 1000 µg/l
Specimen Serum/Plasma/Whole Blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

Serum specimens

  • Vortex 100 µl serum + 100 µl Internal Standard + 100 µl Precipitation Reagent in a reaction vial for 30 s.
  • Incubate 10 min at +2 to +8 °C.
  • Centrifuge 10 min at approx. 16 000 rpm.
  • Inject 20 µl supernatant into the HPLC system.

Plasma specimens

  • For the determination of serotonin in plasma it is necessary to dilute the Internal Standard 1:10 with ultrapure water (HPLC grade).

Whole blood specimens

  • For the step by step description of the sample preparation of whole blood specimens, please refer to the instruction manual.
Run Time < 8 min
Injection Volume 20 µl
Flow Rate 1.0 to 1.2 ml/min
Column Temperature ambient (~25 °C)
Potential approx. 400 to 500 mV
Additional Info

For the HPLC analysis of serotonin any isocratic HPLC system with electrochemical detector is suitable.

Parameters Serotonin
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.