β-Carotene in Serum/Plasma - HPLC

Order No.: 32000, for 100 tests
Parameters:
All-trans-β-Carotene

Fast and easy sample preparation
Low costs per test
High sample stability
Further analytes detectable: Cryptoxanthin, Lycopene, α-Carotene, cis-β-Carotene

All-trans-β-Carotene

Clinical relevance

β-carotene (provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. Like glutathione or vitamin C, it possesses antioxidative properties. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products.

β-carotene can only be processed by the body when absorbed together with fat. Any additional high-dosed preventive therapy should be used with caution to prevent a possible overdose. Additional use of high dose β-carotene over several years combined with nicotine and alcohol consumption may even increase the risk of lung or bowel cancer. For this reason, medications that contain β-carotene are labelled with a warning for smokers. Furthermore, drugs with high concentrations of β-carotene are no longer allowed to be prescribed to smokers either.

 

Product advantages

  • Simple, fast and effective sample preparation
  • High sample stability
  • Low costs per test

 

This reagent kit is designed for easy and reliable determination of β-carotene in serum/plasma. The sample clean up procedure combines a fast precipitation and extraction step. The resulting extract is soluble in the mobile phase, so no evaporation is necessary. The chromatographic determination is run on an isocratic HPLC system with UV/VIS detection. A special RP-chromatography ensures a reliable separation of α-, cis-β-, and all-trans-β-carotene as well as some further carotenoids in less than 10 minutes. The analytes are quantified by the inclusion of an internal standard, which is a non-naturally occurring carotenoid-derivative, so that only a single detection wavelength is required. The high stability of the processed samples allows for easier performance of large sample batches.

 

.

More Information
Number of Tests 100
Limit of quantification 5 ng/ml
Linearity up to 3000 ng/ml
Recovery 107 %
Intraassay

CV ≤ 2.0 %

Interassay

CV ≤ 3.1 %

Analysis Time approx. 10 min
Sample Preparation

 

  • Pipette 100 µl serum/plasma into a light protected reaction vial.
  • Add 50 µl internal standard, mix briefly.
  • Add 50 µl Precipitation Reagent, mix briefly (vortex), do not centrifuge!
  • Add 200 µl Extraction Buffer, mix about 30 s (vortex).
  • Centrifuge 10 min at 16000 x g.
  • Transfer the supernatant into a light protected autosampler vial (glass!).
  • Inject 50 µl into the HPLC system.
Sample Stability The prepared samples are stable for 5 days at room temperature, at +2 to 8 °C up to 1 week (in glass vials and light protected). For longer storage (up to 4 weeks) keep them below -18 °C.
Specimen Serum/Plasma
Pre-analytic Treatment The patient samples are stable at +2 to +8 °C up to 5 days, light-protected in a closed vial. For longer storage (up to 2 months) keep samples below -18 °C. Specimens should be kept frozen and light-protected for transport.
Injection Volume 50 µl
Flow rate 1.5-1.8 ml/min
Column temperature ambient (~25 °C)
Wavelength 453 nm
Gradient Isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter All-trans-β-Carotene
Additional Info

Any isocratic HPLC system with pump, injector and UV detector is suitable.

  1. 32001 HPLC mobile phase ß-carotene serum plasma
    Mobile Phase
    Order no.: 32001
    β-Carotene in Serum/Plasma - HPLC
    Details
  2. 32001 HPLC mobile phase ß-carotene serum plasma
    Mobile Phase, 10 x 1000 ml
    Order no.: 32002
    β-Carotene in Serum/Plasma - HPLC
    Details
  3. 32003 HPLC ß-carotene serum calibration standard
    Beta-Carotene Serum Calibration Standard
    Order no.: 32003
    β-Carotene in Serum/Plasma - HPLC
    Details
  4. 32004 HPLC internal standard ß-carotene serum plasma
    Internal Standard
    Order no.: 32004
    β-Carotene in Serum/Plasma - HPLC
    Details
  5. 32005 HPLC precipitation reagent ß-carotene serum plasma
    Precipitation Reagent
    Order no.: 32005
    β-Carotene in Serum/Plasma - HPLC
    Details
  6. 32006 HPLC extraction buffer ß-carotene serum plasma
    Extraction Buffer
    Order no.: 32006
    β-Carotene in Serum/Plasma - HPLC
    Details
  7. Reaction Vials, 1.5 ml
    Reaction Vials, 1.5 ml
    Order no.: 33005
    Amber colour, light protected
    Details
  1. 32003 HPLC ß-carotene serum calibration standard
    Beta-Carotene Serum Calibration Standard
    Order no.: 32003
    β-Carotene in Serum/Plasma - HPLC
    Details
  1. 0025 0026 0027 HPLC β-carotene serum controls
    β-Carotene Serum Controls
    Order no.: 0025/0026/0027
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. 32100 HPLC column ß-carotene serum plasma
    HPLC column
    Order no.: 32100
    β-Carotene in Serum/Plasma
    Details
  3. PEEK-encased Prefilter, 5 µm
    Details
  4. Precolumn Cartridge Holder 4/10
    Details
  5. Precolumn cartridge 4/10
    Precolumn cartridge 4/10
    Order no.: 18032
    β-Carotene in Serum/Plasma - HPLC
    Details
As a customer please login or register to gain full access.