Parameter Set Mycophenolic Acid - LC-MS/MS

Order No.: 92916/RUO
Parameters:
Mycophenolic Acid, Mycophenolic Acid Glucuronide

For the analysis of MPA and MPAG
6PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

Mycophenolic Acid

Mycophenolic Acid Glucuronide

Clinical relevance

This parameter set allows the quantitative determination of mycophenolic acid and its glucuronide in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 1.8 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2

Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Mycophenolic Acid, Mycophenolic Acid Glucuronide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

Mycophenolic Acid

Mycophenolic Acid Glucuronide

Clinical relevance

This parameter set allows the quantitative determination of mycophenolic acid and its glucuronide in human serum or plasma samples via liquid chromatography mass spectrometry (LC-MS/MS).

 

MassTox® Series A

The MassTox® Series A is a modular system that enables the determination of all analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® Basic Kit A
• Specific MassTox® Parameter Set
• Analytical column MassTox® TDM MasterColumn® A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 1.8 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2

Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Mycophenolic Acid, Mycophenolic Acid Glucuronide
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: