MassChrom® Amino Acid Analysis in Plasma/Serum with 96 Deep Well Plate

Order No.: 75111/DWP/RUO, for 3 x 96 tests
Parameters:
Amino Acids

Quantitative analysis of 48 amino acids
No derivatisation
Short run times
Excellent separation of isobaric analytes
Each analyte safeguarded by its own internal standard

Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Leucine
Isoleucine
Allo-Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

The 7 analytes highlighted in red can be measured with both the full panel and the specific panel.

This assay is designed for the quantitative determination of amino acids in plasma/serum by LC-MS/MS. The method covers 48 amino acids and derivatives that are analysed within 20 min in one single run. Alternatively, the same assay allows the determination of 7 specific analytes in a much shorter run time (8-9 min) using a modified gradient. Both panels use the identical chromatographic set up, research sample preparation procedure, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls. In addition, each of the 48 analytes is safeguarded by an own internal standard. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.

More Information
Number of Tests 3 x 96
Sample Preparation
  • Pipette 50 μl reconstituted Internal Standard Mix
    + 25 μl research sample/calibrator/MassCheck® control
    + 400 μl Precipitation Reagent into a 96 deep well plate.
  • Shake for 2 min at 1200 rpm and centrifuge for 5 min at 2000 x g.
  • Transfer supernatant into collection plate and seal with pierceable heat seal.
  • Inject 2–5 μl into the LC-MS/MS system.
Sample Stability Stability of research samples depends on the specific analyte. Further information can be obtained from the instruction manual.
Specimen Plasma/Serum
Injection Volume 2–5 μl
Column temperature ambient (~ 25 °C)
Gradient

Full panel: binary gradient, flow gradient
Specific panel: isocratic

Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Amino Acids
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 75001/RUO

    MasChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 75002/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  3. Rinsing Solution
    Rinsing Solution
    Order no.: 75009/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  4. Collection Plates
    Collection Plates
    Order no.: 75058/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  5. Pierceable Heat Seals for 96 Well Plates
    Pierceable Heat Seals for 96 Well Plates
    Order no.: 75060/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  6. Precipitation Reagent
    Precipitation Reagent
    Order no.: 75105/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  7. Internal Standard Set Amino Acid Analysis
    Internal Standard Set Amino Acid Analysis
    Order no.: 75146/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  8. 96 Deep Well Plates
    96 Deep Well Plates
    Order no.: 75156/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  1. Antibiotics Plasma Controls 0182 0183 0184
    MassCheck® Amino Acid Analysis Plasma Controls
    Order no.: 0471/0472/0473

    Order no.: 0471/0472/0473

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    Details
  3. System Check Solution
    System Check Solution
    Order no.: 75010/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  4. Tuning Mix 1 Amino Acid Analysis
    Tuning Mix 1 Amino Acid Analysis
    Order no.: 75015/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  5. Tuning Mix 2 Amino Acid Analysis
    Tuning Mix 2 Amino Acid Analysis
    Order no.: 75016/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  6. Tuning Mix 3 Amino Acid Analysis
    Tuning Mix 3 Amino Acid Analysis
    Order no.: 75017/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  7. Tuning Mix 4 Amino Acid Analysis
    Tuning Mix 4 Amino Acid Analysis
    Order no.: 75018/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details
  8. Tuning Mix 5 Amino Acid Analysis
    Tuning Mix 5 Amino Acid Analysis
    Order no.: 75019/RUO

    MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS

    Details