MassChrom® Amino Acid Analysis in Plasma/Serum with 96 Deep Well Plate - LC-MS/MS

Order No.: 75111/DWP/RUO, for 3 x 96 tests
Parameters:
Amino Acids

Quantitative analysis of 48 amino acids
No derivatisation
Short run times
Excellent separation of isobaric analytes
Each analyte safeguarded by its own internal standard

Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Leucine
Isoleucine
Allo-Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

The 7 analytes highlighted in red can be measured with both the full panel and the specific panel.

The New Standard in Amino Acid Analysis

  • Quantitative Amino Acid Analysis (AAA) of 48 analytes
  • Fast sample prep without derivatisation
  • Short run times
  • Excellent separation of isobaric analytes
  • Each analyte safeguarded by its own internal standard

MassChrom® Amino Acid Analysis (AAA) is designed for the quantitative determination of amino acids in plasma/serum by LC-MS/MS. The method covers 48 amino acids and derivatives that are analysed within 20 min in one single run. Alternatively, the same assay allows the determination of 7 specific analytes in a much shorter run time (8-9 min) using a modified gradient. Both panels use the identical chromatographic setup, research sample preparation procedure, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls. In addition, each of the 48 analytes is safeguarded by an own internal standard.

The assay is available in 2 versions either with reaction vials or with 96 deep well plates.

No Derivatisation? - No Problem! 

All HPLC assays and most LC-MS/MS assays require derivatisation steps for a good separation of isobaric analytes but to also increase detector response. In contrast, the MassChrom® LC-MS/MS assay eliminates the need for derivatisation.
The benefits:

  • Faster sample prep
  • Error-prone analytical steps are omitted
  • Increased accuracy of results and higher robustness of the method

Excellent Separation of Isobaric Analytes

A number of amino acids are isobars and require a clear chromatographic separation. The sophisticated chromatographic setup of MassChrom® Amino Acid Analysis provides an efficient separation of all isobars such as Ile, Leu and Allo-Ile and works reliably for both, full panel and specific panel.

100% Coverage - 48 isotopically-labelled Internal Standards

Each of the 48 amino acids is safeguarded by its own isotopically-labelled internal standard. This includes also saccharopine and hydroxylysine that are custom-made and commercially not available anywhere else. The internal standards take care of a potential small variability in recovery, matrix effects and ionisation effectiveness by providing virtually identical chemical properties of the respective analyte.

Matrix-based Quality Controls and Calibrators

Quality controls and the 3PLUS1® Multilevel Calibrator Set are human-matrix based. This is aimed at simulating the composition of samples as closely as possible. It reflects the performance of the method with real samples and helps to minimise matrix effects.

The calibrator material from Chromsystems is also linked to a common reference, a standard of higher order, documented in a traceability chain and available on the website. Customers can also download an Excel file with the values for quality controls and calibrators, eliminating the need for manual data entry. 

Short Run Times 

Standard HPLC Amino Acid Analysers use ion exchange chromatography and derivatisation with ninhydrin, requiring approximately 140-150 min for the analysis of all compounds. In contrast,  the LC-MS/MS assay from Chromsystems requires less than 20 minutes to quantify all 48 amino acids in a single run. This translates in a 7-fold increase of throughput in the laboratory. The speed of analysis can be further increased by using the shorter specific panel with a throughput of more than 17 samples within the same time frame of one analytical run with a standard HPLC-based assay. A switch between these 2 modes requires no changes to the chromatographic setup and can therefore be chosen flexibly depending on the sample amount and requirements.

This assay is designed for the quantitative determination of amino acids in plasma/serum by LC-MS/MS. The method covers 48 amino acids and derivatives that are analysed within 20 min in one single run. Alternatively, the same assay allows the determination of 7 specific analytes in a much shorter run time (8-9 min) using a modified gradient. Both panels use the identical chromatographic set up, research sample preparation procedure, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls. In addition, each of the 48 analytes is safeguarded by an own internal standard. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.

More Information
Method of Analysis HPLC
Number of Tests 3 x 96
Specimen Plasma/Serum
Sample Preparation
  • Pipette 50 μl reconstituted Internal Standard Mix
    + 25 μl research sample/calibrator/MassCheck® control
    + 400 μl Precipitation Reagent into a 96 deep well plate.
  • Shake for 2 min at 1200 rpm and centrifuge for 5 min at 2000 x g.
  • Transfer supernatant into collection plate and seal with pierceable heat seal.
  • Inject 2–5 μl into the LC-MS/MS system.
Sample Stability Stability of research samples depends on the specific analyte. Further information can be obtained from the instruction manual.
Injection Volume 2–5 μl
Column temperature ambient (~ 25 °C)
Gradient

Full panel: binary gradient, flow gradient
Specific panel: isocratic

Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Amino Acids