Antiepileptic Drugs in Serum/Plasma, HIGH RESOLUTION - HPLC

Order No.: 22000/HR/RUO, for 100 tests
Parameters:
10-OH-Carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine, Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide

All relevant drugs in one run
Fast sample preparation

Carbamazepine

10-OH-carbamazepine

Carbamazepine-10,11-epoxide

Oxcarbamazepine

Ethosuximide

Lamotrigine

Phenobarbital

Phenytoin

Primidone

Sultiame

Zonisamide

This Chromsystems reagent kit is designed for the specific determination of the antiepileptic drugs carbamazepine, oxcarbamazepine, ethosuximide, lamotrigine, primidone, phenobarbital, phenytoin, sultiame and zonisamide in serum or plasma. In addition, the analogues carbamazepine-10,11-epoxide, 10,11-dihydro-10,11-dihydroxycarbamazepine, 10-OH-carbamazepine and 2-ethyl-2-phenylmalondiamide (PEMA) can be analysed.
Sample preparation is based on a simple but efficient precipitation step. To separate and quantify all analytes a chromatography with high resolution is used.

More Information
Number of Tests 100
Analysis Time High Resolution: 22 min
Sample Preparation
  • Pipette 100 μl serum/plasma (standards, controls, research specimens) and 150 μl Internal Standard into a labelled reaction vial, mix briefly (vortex).
  • Add 50 μl Precipitation Reagent, mix 1 min (vortex).
  • Centrifuge 10–15 min at 9000 x g.
  • Mix 100 μl of the supernatant with 100 μl Stabilisation Buffer.
  • Inject 20 μl into the HPLC system.
Sample Stability The research samples can be kept refrigerated (+2 to +8°C) for approx. one week. For longer storage (up to 2 weeks) deep-freeze them below –18°C.
Specimen Serum/Plasma
Injection Volume 20 µl
Flow rate High Resolution: 1.2 ml/min
Column temperature ambient (~25 °C)
Wavelength 204 nm
Information

A precolumn has to be used.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter 10-OH-Carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine, Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide
Additional Info Analysis of antiepileptic drugs requires a simple isocratic HPLC system with HPLC pump, injector and UV detector. The use of a thermostat-controlled column oven avoids temperature variations and optimises the stability of the separation.
  1. Mobile Phase, High Resolution
    Mobile Phase, High Resolution
    Order no.: 22001/HR/RUO

    Antiepileptic Drugs in Serum/Plasma -  HPLC

    Details
  2. Precipitation Reagent
    Precipitation Reagent
    Order no.: 22003/RUO

    Antiepileptic Drugs in Serum/Plasma - HPLC

    Details
  3. Internal Standard
    Internal Standard
    Order no.: 22004/RUO

    Antiepileptic Drugs in Serum/Plasma - HPLC

    Details
  4. Stabilisation Buffer
    Stabilisation Buffer
    Order no.: 22006/RUO

    Antiepileptic Drugs in Serum/Plasma - HPLC

    Details
  5. Reaction vials
    Reaction vials
    Order no.: 3006/RUO
     
    Details
  1. 0060 0160 0070 0170 0080 0166 0188 HPLC AED antiepileptic drugs serum controls
    AED Serum Controls
    Order no.: 0060/0160/0070/0170/0080/0180/0166/0188
    Details
  1. HPLC column, High Resolution
    HPLC column, High Resolution
    Order no.: 22100/HR/RUO

    Antiepileptic Drugs in Serum/Plasma

    Details
  2. PEEK-encased prefilter, 5 µm
    PEEK-encased prefilter, 5 µm
    Order no.: 15009/RUO
     
    Details
  3. PEEK prefilter housing
    PEEK prefilter housing
    Order no.: 15010/RUO
     
    Details
  4. Precolumn cartridge holder 4/10
    Precolumn cartridge holder 4/10
    Order no.: 18001/RUO
     
    Details
  5. Precolumn 4/10
    Precolumn 4/10
    Order no.: 18022/F/HR/RUO

    Antiepileptic Drugs in Serum/Plasma - HPLC

    Details