MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
Isotopically labelled internal standard
Multilevel calibrators
25-OH-Vitamin D2
25-OH-Vitamin D3
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS
Kit for 200 tests (Order no. 62000/RUO)
Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls (Order no. 62000/1000/RUO)
This assay allows the quantitative determination of 25-hydroxycholecalciferol (25-OH-vitamin D3) and 25-hydroxyergocalciferol (25-OH-vitamin D2) in human plasma or serum samples.
Manual sample preparation is limited to simple and effective protein precipitation. A trap column concentrates the analytes and separates interfering substances. A two-position-six-port-valve connects the trap column to an HPLC column where the chromatographic separation takes place. The use of atmospheric pressure chemical ionisation (APCI) and a deuterated internal standard assures precision and robustness and minimises ion suppression effects.
This kit does not separate 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2 from 25-OH-vitamin D3 and 25-OH-vitamin D2, respectively, and should therefore only be used to analyse samples that contain none or just negligible amounts of these metabolites.
We recommend the use of the assay MassChrom® 25-OH-Vitamin D3/D2 and 3-epi-25-OH-Vitamin D3 in Serum/Plasma (order no. 62062/RUO) for chromatographic separation of the diastereomers and quantitation of 3-epi-25-OH-vitamin D3 to ensure correct assessment of the vitamin D status in samples that might contain 3-epi-25-OH-vitamin D3/D2.
Content for 200 tests (62000/RUO)
Mobile Phase A, 1000 ml (1x)
Mobile Phase B, 1000 ml (1x)
Precipitation Reagent, 5 ml (1x)
Internal Standard Mix, 40 ml (1x)
Rinsing Solution, 1000 ml (1x)
Reaction Vials, 100 pcs. (2x)
Content for 1000 tests (62000/1000/RUO)
Mobile Phase A, 1000 ml (5x)
Mobile Phase B, 1000 ml (5x)
Precipitation Reagent, 5 ml (5x)
Internal Standard, 40 ml (5x)
Rinsing Solution, 1000 ml (2x)
Reaction Vials, 100 pcs. (10x)
3PLUS1® 25-OH-Vitamin D3/D2 Multilevel Serum Calibrator Set (lyoph.), 1 ml (4x)
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level I (lyoph.), 1 ml (5x)
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level II (lyoph.), 1 ml (5x)
Analytical Column, 1 pc. (1x)
Trap Column, 1 pc. (2x)
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual. |
| Specimen | Serum/Plasma |
| Sample Preparation |
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| Run Time | 5.0 or 6.0 min (depending on gradient system) |
| Injection Volume | ≤ 50 µl (mass spectrometer dependent) |
| Column Temperature | Analytical Column and Trap Column: +20 to +25 °C |
| Ionisation | APCI positive |
| MS/MS Mode | MRM |
| Parameters | 25-OH-Vitamin D2, 25-OH-Vitamin D3 |
| Order No. | MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS |
| 62000/RUO | Kit for 200 tests |
| 62000/1000/RUO | Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls |
| Order No. | Kit components (Quantities in kit for 200 tests) |
| 62001/RUO | Mobile Phase A, 1000 ml (1x) |
| 62002/RUO | Mobile Phase B, 1000 ml (1x) |
| 62003/RUO | Precipitation Reagent, 5 ml (1x) |
| 62004/RUO | Internal Standard, 40 ml (1x) |
| 62009/RUO | Rinsing Solution, 1000 ml (1x) |
| 3006 | Reaction Vials, 100 pcs. (2x) |
| Order No. | Required components (not included in the kit for 200 tests) |
| 62100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 62110/RUO | Trap Column, equilibrated, with test chromatogram, 1 pc. |
| 62028 | 3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 4 x 1 ml (lyoph.) |
| 62039 | 6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 7 x 1 ml (lyoph.) |
| 0222 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level I, 5 x 1 ml (lyoph.) |
| 0223 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level II, 5 x 1 ml (lyoph.) |
| 0256 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level III, 5 x 1 ml (lyoph.) |
| 62015/RUO | Tuning Mix 25-OH-Vitamin D3/D2, Analytes and Internal Standards, 2 ml |
| Order No. | General lab equipment (non-CE/IVD products) |
| 3006 | Reaction Vials, 100 pcs. |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15011 | PEEK-encased Prefilter, 2 µm, 5 pcs. |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 0,5 µm, 5 pcs. |
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Precipitation ReagentOrder no.: 62003/RUO
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62028MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS -
6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62039MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS -
MassCheck® 25-OH-Vitamin D3/D2 Serum ControlsOrder no.: 0221/0222/0223/0256MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS
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Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015/RUO
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62028MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62039MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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MassCheck® 25-OH-Vitamin D3/D2 Serum ControlsOrder no.: 0221/0222/0223/0256
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
25-OH-Vitamin D2
25-OH-Vitamin D3
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS
Kit for 200 tests (Order no. 62000/RUO)
Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls (Order no. 62000/1000/RUO)
This assay allows the quantitative determination of 25-hydroxycholecalciferol (25-OH-vitamin D3) and 25-hydroxyergocalciferol (25-OH-vitamin D2) in human plasma or serum samples.
Manual sample preparation is limited to simple and effective protein precipitation. A trap column concentrates the analytes and separates interfering substances. A two-position-six-port-valve connects the trap column to an HPLC column where the chromatographic separation takes place. The use of atmospheric pressure chemical ionisation (APCI) and a deuterated internal standard assures precision and robustness and minimises ion suppression effects.
This kit does not separate 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2 from 25-OH-vitamin D3 and 25-OH-vitamin D2, respectively, and should therefore only be used to analyse samples that contain none or just negligible amounts of these metabolites.
We recommend the use of the assay MassChrom® 25-OH-Vitamin D3/D2 and 3-epi-25-OH-Vitamin D3 in Serum/Plasma (order no. 62062/RUO) for chromatographic separation of the diastereomers and quantitation of 3-epi-25-OH-vitamin D3 to ensure correct assessment of the vitamin D status in samples that might contain 3-epi-25-OH-vitamin D3/D2.
Content for 200 tests (62000/RUO)
Mobile Phase A, 1000 ml (1x)
Mobile Phase B, 1000 ml (1x)
Precipitation Reagent, 5 ml (1x)
Internal Standard Mix, 40 ml (1x)
Rinsing Solution, 1000 ml (1x)
Reaction Vials, 100 pcs. (2x)
Content for 1000 tests (62000/1000/RUO)
Mobile Phase A, 1000 ml (5x)
Mobile Phase B, 1000 ml (5x)
Precipitation Reagent, 5 ml (5x)
Internal Standard, 40 ml (5x)
Rinsing Solution, 1000 ml (2x)
Reaction Vials, 100 pcs. (10x)
3PLUS1® 25-OH-Vitamin D3/D2 Multilevel Serum Calibrator Set (lyoph.), 1 ml (4x)
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level I (lyoph.), 1 ml (5x)
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level II (lyoph.), 1 ml (5x)
Analytical Column, 1 pc. (1x)
Trap Column, 1 pc. (2x)
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual. |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | 5.0 or 6.0 min (depending on gradient system) |
| Injection Volume | ≤ 50 µl (mass spectrometer dependent) |
| Column Temperature | Analytical Column and Trap Column: +20 to +25 °C |
| Ionisation | APCI positive |
| MS/MS Mode | MRM |
| Parameters | 25-OH-Vitamin D2, 25-OH-Vitamin D3 |
| Order No. | MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS |
| 62000/RUO | Kit for 200 tests |
| 62000/1000/RUO | Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls |
| Order No. | Kit components (Quantities in kit for 200 tests) |
| 62001/RUO | Mobile Phase A, 1000 ml (1x) |
| 62002/RUO | Mobile Phase B, 1000 ml (1x) |
| 62003/RUO | Precipitation Reagent, 5 ml (1x) |
| 62004/RUO | Internal Standard, 40 ml (1x) |
| 62009/RUO | Rinsing Solution, 1000 ml (1x) |
| 3006 | Reaction Vials, 100 pcs. (2x) |
| Order No. | Required components (not included in the kit for 200 tests) |
| 62100 | Analytical Column, equilibrated, with test chromatogram, 1 pc. |
| 62110/RUO | Trap Column, equilibrated, with test chromatogram, 1 pc. |
| 62028 | 3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 4 x 1 ml (lyoph.) |
| 62039 | 6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 7 x 1 ml (lyoph.) |
| 0222 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level I, 5 x 1 ml (lyoph.) |
| 0223 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level II, 5 x 1 ml (lyoph.) |
| 0256 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control Level III, 5 x 1 ml (lyoph.) |
| 62015/RUO | Tuning Mix 25-OH-Vitamin D3/D2, Analytes and Internal Standards, 2 ml |
| Order No. | General lab equipment (non-CE/IVD products) |
| 3006 | Reaction Vials, 100 pcs. |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15011 | PEEK-encased Prefilter, 2 µm, 5 pcs. |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15071 | Stainless Steel Prefilter, 0,5 µm, 5 pcs. |
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Precipitation ReagentOrder no.: 62003/RUO
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62028MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62039MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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MassCheck® 25-OH-Vitamin D3/D2 Serum ControlsOrder no.: 0221/0222/0223/0256
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS
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Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015/RUO
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62028MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2Order no.: 62039MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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MassCheck® 25-OH-Vitamin D3/D2 Serum ControlsOrder no.: 0221/0222/0223/0256
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS