Vitamin C in Plasma/Serum - Automated HPLC

Order No.: 65765/F, for 480 tests
Parameters:
Vitamin C

Automated sample preparation with 96 well filter plates

Vitamin C

Clinical relevance

Vitamin C (ascorbic acid, ascorbate) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

In the case of vitamin C deficiency, procollagen chains are produced insufficiently and stable fibrils cannot be formed in the affected cells. Without functioning collagen, the blood vessels and tendons become fragile and the typical skin-related symptoms associated with scurvy start to develop. In addition to the collagen damages described above, further diseases caused by vitamin C deficiency include bleeding of uncertain origin, loss of teeth and overactive thyroid gland (hyperthyroidism). Vitamin C deficiency is also observed in dialysis patients, in chronic alcohol abuse and during pregnancy.

 

Product advantages

  • High stability of processed samples and controls
  • Enhanced column stability
  • Reliable quantification with a stable internal standard

 

This Chromsystems kit allows the reliable chromatographic determination of vitamin C in one isocratic HPLC run with UV detection. An efficient protein precipitation step removes interfering components and stabilises Vitamin C simultaneously. A stable internal standard is used for reliable quantification. Due to a special stabilisation of the prepared samples, it is also possible to reliably process and measure large sample batches.

 

Kit content

Mobile Phase, 1000 ml
Vitamin C Plasma Calibration Standard (lyoph.), 5 x 0.5 ml
Internal Standard, 10 ml
96 Well filter plates, 5 pcs.
Collection plates, 5 pcs.
Pierceable adhesive seals for 96 well plates, 5 pcs.

More Information
Number of Tests 480
Limit of quantification

0.5 mg/l

Linearity up to 120 mg/l
Intraassay

CV <= 3 %

Interassay CV < 6 %
Analysis Time 5 min
Sample Preparation
  • Place 100 µl Internal Standard in a 96 well filter plate.
  • Add 100 µl sample/calibrator/control.
  • Vortex for 30 s at 1100 rpm (orbit: 2 mm).
  • Centrifuge for 5 min at 2000 x g.
  • Close the 96 well filter plate.
  • Vortex for 30 s at 800 rpm (orbit: 2 mm).
  • Inject 20 µl of the prepared sample into the HPLC system.
Specimen Plasma/Serum
Pre-analytic Treatment

Specimens: Fresh lithium heparin plasma

Sample stability: 5 days at -20 °C, up to 2 hours at room temperature
Detailed information on stability and transportation can be obtained from the instruction manual.

Injection Volume 20 µl
Flow rate 1.5 ml/min
Column temperature ambient (~25 °C)
Wavelength 245 nm
Gradient Isocratic
Information Any isocratic HPLC system with UV detection is suitable.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Vitamin C
Additional Info

For the Chromsystems HPLC analysis of vitamin C any isocratic HPLC system with UV detection is suitable.

Flow 1.5 ml/min
  1. 65001 HPLC mobile phase vitamin C plasma serum
    Mobile Phase
    Order no.: 65001
    Vitamin C in Plasma/Serum - HPLC
    Details
  2. 65001 HPLC mobile phase vitamin C plasma serum
    Mobile Phase, 10 x 1000ml
    Order no.: 65002
    Vitamin C in Plasma/Serum - HPLC
    Details
  3. 65003 HPLC plasma calibration standard vitamin C
    Vitamin C Plasma Calibration Standard
    Order no.: 65003
    Vitamin C in Plasma/Serum - HPLC
    Details
  4. 65044 HPLC internal standard vitamin C plasma serum
    Internal Standard
    Order no.: 65044
    Vitamin C in Plasma/Serum - HPLC
    Details
  5. 96 Well Filter Plates
    96 Well Filter Plates
    Order no.: 65757
    Vitamin C in Plasma/Serum – automated HPLC
    Details
  6. Collection Plates
    Collection Plates
    Order no.: 65758
    Vitamin C in Plasma/Serum – automated HPLC
    Details
  7. Pierceable adhesive seals for 96 Well Plates
    Pierceable adhesive seals for 96 Well Plates
    Order no.: 65759
    Vitamin C in Plasma/Serum – automated HPLC
    Details
  1. 65003 HPLC plasma calibration standard vitamin C
    Vitamin C Plasma Calibration Standard
    Order no.: 65003
    Vitamin C in Plasma/Serum - HPLC
    Details
  1. 0074 0075 0076 HPLC vitamin C plasma controls
    Vitamin C Plasma Controls
    Order no.: 0074/0075/0076
    Details
  1. PEEK prefilter housing
    PEEK prefilter housing
    Order no.: 15010
     
    Details
  2. 65100 HPLC column vitamin C plasma serum
    HPLC column
    Order no.: 65100
    Vitamin C in Plasma/Serum - HPLC
    Details
  3. PEEK-encased prefilter, 5 µm
    Details
  4. Precolumn cartridge 4/10
    Precolumn cartridge 4/10
    Order no.: 18065
    Vitamin C in Plasma/Serum - HPLC
    Details
  5. Precolumn cartridge holder 4/10
    Details
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