Vitamin C in Plasma/Serum - Automated HPLC
Automated sample preparation with 96 well filter plates
Vitamin C (ascorbic acid, ascorbate) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.
In the case of vitamin C deficiency, procollagen chains are produced insufficiently and stable fibrils cannot be formed in the affected cells. Without functioning collagen, the blood vessels and tendons become fragile and the typical skin-related symptoms associated with scurvy start to develop. In addition to the collagen damages described above, further diseases caused by vitamin C deficiency include bleeding of uncertain origin, loss of teeth and overactive thyroid gland (hyperthyroidism). Vitamin C deficiency is also observed in dialysis patients, in chronic alcohol abuse and during pregnancy.
- High stability of processed samples and controls
- Enhanced column stability
- Reliable quantification with a stable internal standard
This Chromsystems kit allows the reliable chromatographic determination of vitamin C in one isocratic HPLC run with UV detection. An efficient protein precipitation step removes interfering components and stabilises Vitamin C simultaneously. A stable internal standard is used for reliable quantification. Due to a special stabilisation of the prepared samples, it is also possible to reliably process and measure large sample batches.
Mobile Phase, 1000 ml
Vitamin C Plasma Calibration Standard (lyoph.), 5 x 0.5 ml
Internal Standard, 10 ml
96 Well filter plates, 5 pcs.
Collection plates, 5 pcs.
Pierceable adhesive seals for 96 well plates, 5 pcs.
|Number of Tests||480|
|Limit of quantification||
|Linearity||up to 120 mg/l|
CV <= 3 %
|Interassay||CV < 6 %|
|Analysis Time||5 min|
Specimens: Fresh lithium heparin plasma
Sample stability: 5 days at -20 °C, up to 2 hours at room temperature
|Injection Volume||20 µl|
|Flow rate||1.5 ml/min|
|Column temperature||ambient (~25 °C)|
|Information||Any isocratic HPLC system with UV detection is suitable.|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Method of Analysis||HPLC|
For the Chromsystems HPLC analysis of vitamin C any isocratic HPLC system with UV detection is suitable.