MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard, plus the multilevel calibrators (6PLUS1®, 3PLUS1®) assures high performance and minimises ion suppression effects.

An upgrade for the determination of the epimeric forms of 25-OH-Vitamin D₃ and 25-OH-Vitamin D₂ is also available for this kit (see accessories).

Kit content 62000/RUO                                

Mobile Phase A, 1000 ml                     
Mobile Phase B, 1000 ml                      
Precipitation Reagent, 5 ml                 
Internal Standard, 40 ml                        
Rinsing Solution, 1000 ml                    
Reaction vials, 2 x 100 pcs.        

Kit content 62000/1000/RUO   

Mobile Phase A, 5 x 1000 ml                                                                   
Mobile Phase B, 5 x 1000 ml                                                                  
Precipitation Reagent, 5 x 5 ml                                                                
Internal Standard, 5 x 40 ml                                                                    
Rinsing Solution, 2 x 1000 ml                                                        
Reaction vials, 10 x 100 pcs.        
Analytical column, 1 pc.           
Trap column, 2 pcs.                   
3PLUS1^® 25-OH-Vitamin D₃/D₂ Multilevel Serum  Calibrator Set (lyoph.), 4 x 1 ml            
MassCheck^® 25-OH-Vitamin D₃/D₂ Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck^® 25-OH-Vitamin D₃/D₂ Serum Control, Level II (lyoph.), 5 x 1 ml