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Parameter Set Antimycotic Drugs - LC-MS/MS

Order No.: 92922/RUO
Parameters:
5-Flucytosine, Fluconazole, Hydroxyitraconazole, Itraconazole, Ketoconazole, Posaconazole, Voriconazole

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

main product photo

5-Flucytosine

Fluconazole

Itraconazole

Hydroxyitraconazole

Ketoconazole

Posaconazole

Voriconazole

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antimycotic Drugs allows for the fast quantitative determination of fluconazole, 5-flucytosine, hydroxyitraconazole, itraconazole, ketoconazole, posaconazole and voriconazole in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

More Information
Analysis Time 3.2 min
Sample Preparation
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Sample Stability Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient
0.00 - 0.50 min 30 % Mobile Phase 2
0.51 - 2.80 min 100 % Mobile Phase 2
2.81 - 3.20 min 30 % Mobile Phase 2
Ionisation ESI positive
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 5-Flucytosine, Fluconazole, Hydroxyitraconazole, Itraconazole, Ketoconazole, Posaconazole, Voriconazole
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    3PLUS1® Multilevel Plasma Calibrator Set Antimycotic Drugs
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    Order no.: 0252/0253/0254

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    Analytical column for MassTox® Series A

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    Order no.: 92039/RUO

    Tuning Mix for the Parameter Set Antimycotic Drugs

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  4. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
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