Parameter Set Antimycotic Drugs - LC-MS/MS

Order No.: 92922/RUO
Parameters:
5-Flucytosine, Fluconazole, Hydroxyitraconazole, Itraconazole, Ketoconazole, Posaconazole, Voriconazole

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

5-Flucytosine

Fluconazole

Itraconazole

Hydroxyitraconazole

Ketoconazole

Posaconazole

Voriconazole

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antimycotic Drugs allows for the fast quantitative determination of fluconazole, 5-flucytosine, hydroxyitraconazole, itraconazole, ketoconazole, posaconazole and voriconazole in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

More Information
Analysis Time 3.2 min
Sample Preparation
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Sample Stability Stability of research samples depends on the specific analyte. Further information can be optained from the instruction manual.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient
0.00 - 0.50 min 30 % Mobile Phase 2
0.51 - 2.80 min 100 % Mobile Phase 2
2.81 - 3.20 min 30 % Mobile Phase 2
Ionisation ESI positive
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 5-Flucytosine, Fluconazole, Hydroxyitraconazole, Itraconazole, Ketoconazole, Posaconazole, Voriconazole
  1. 0252 0253 0254 LCMS TDM Series A controls antimycotic drugs
    MassCheck® Antimycotic Drugs Plasma Controls
    Order no.: 0252/0253/0254

    Order no.: 0252/0253/0254

    Details
  2. Internal Standard Mix
    Internal Standard Mix
    Order no.: 92644/RUO
    Component of the Parameter Set Antimycotic Drugs, available separately
    Details
  1. 0252 0253 0254 LCMS TDM Series A controls antimycotic drugs
    MassCheck® Antimycotic Drugs/EXTENDED Plasma Controls
    Order no.: 0252/XT; 0253/XT; 0254/XT

    Order no.: 0252/0253/0254

    Details
  2. 0252 0253 0254 LCMS TDM Series A controls antimycotic drugs
    MassCheck® Antimycotic Drugs Plasma Controls
    Order no.: 0252/0253/0254

    Order no.: 0252/0253/0254

    Details
  1. 92110 TDM Series A Master Column
    MassTox® MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A

    Details
  2. Tuning Mix Antimycotic Drugs
    Tuning Mix Antimycotic Drugs
    Order no.: 92039/RUO

    Tuning Mix for the Parameter Set Antimycotic Drugs

    Details
  3. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  4. PEEK-encased Prefilter, 2 µm
    Details