One Step Vitamins A and E in Serum/Plasma - HPLC

Order No.: 34000/Premix, for 100 tests
Parameters:
Vitamin A, Vitamin E

With ready-to-use pre-mixed tubes
Only one pipetting step
Fully automated workflow available

Vitamin A

Vitamin E

Clinical relevance

Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.

Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.

Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.

However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.

 

Product advantages

  • Extremely simple sample preparation in a single step
  • Easy handling
  • Low analysis costs
  • Automated version available

 

This Chromsystems kit allows the simultaneous analysis of vitamins A and E in serum/plasma in a single isocratic HPLC run with UV detection. The special kit feature: Sample preparation is performed very fast and simply in one single pipetting step using premixed tubes. Additionally, a stable internal standard is used for precise quantification.

Chromsystems offers additional kits for the fast and efficient determination of vitamins A and E. You can choose between several options depending on the device capabilities (HPLC or UHPLC) and the sample quantities in your laboratory. Alternatively, the standard kit 34000 is available, as well as the 34900/UHPLC kit for higher sample throughput using UHPLC determination. Our kits are also available with automated workflow (34700/F and 34900/F).

More Information
Number of Tests 100
Limit of quantification Vitamin A: 0.02 mg/l
Vitamin E: 0.60 mg/l
Linearity

Vitamin A: up to 4.5 mg/l
Vitamin E: up to 38 mg/l

Recovery Vitamin A > 99 %; Vitamin E > 96 %
Intraassay

Vitamin A: CV ≤ 4.9 %

Vitamin E: CV ≤ 4.1 %

Interassay

Vitamin A: CV ≤ 5.2 %

Vitamin E: CV ≤ 3.8 %

Analysis Time approx. 9 min
Sample Preparation
  • Pipette 100 µl serum/plasma into a Pre-mixed Reaction Tube.
  • Mix 30 s (vortex)
  • Centrifuge 10 min at at least 9000 x g.
  • Transfer supernatant into a light protected autosampler vial
  • inject 50 µl into the HPLC system.
Sample Stability Protect prepared samples from light. At +2 to +8°C stable up to 48 h and at +20 to +25 °C up to 24 h. For longer storage (max. 3 weeks) keep the samples below -18 °C.
Specimen Serum/Plasma
Pre-analytic Treatment

Time of sample drawing

  • In the morning before breakfast
  • Prior to any medication

Collection and storage of specimens

  • Collect about 1 to 2 ml blood.
  • Centrifugate 5 min at 2000 x g.
  • Store serum/plasma below -18 °C and protect from light until analysed.
Injection Volume 50 µl
Flow rate 1.50 ml/min
Column temperature ambient (~ 25 °C)
Wavelength

325 nm, after 3.5 min switch to 295 nm

Gradient Isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Vitamin A, Vitamin E
Additional Info

For the analysis of vitamins A and E any isocratic HPLC system with UV detector is suitable. As these vitamins are detected at different wavelengths, a detector with programmable wavelength switching is very useful for simultaneous analysis of vitamins A and E in a single HPLC run.

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    Mobile Phase
    Order no.: 34001
    Vitamins A and E in Serum/Plasma - HPLC
    Details
  2. 34001 HPLC vitamin A vitamin E serum plasma mobile phase
    Mobile Phase, 10 x 1000 ml
    Order no.: 34002
    Vitamins A and E in Serum/Plasma - HPLC
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  3. 34004 vitamin A vitamin E serum plasma calibration standard
    Vitamins A and E Serum Calibration Standard
    Order no.: 34004
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  4. 34906 HPLC vitamin A vitamin E serum plasma premix tubes
    Pre-mixed Reaction Tubes
    Order no.: 34906
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    Details
  1. 34004 vitamin A vitamin E serum plasma calibration standard
    Vitamins A and E Serum Calibration Standard
    Order no.: 34004
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  1. 0032 0036 0037 HPLC vitamin A vitamin E serum controls
    Vitamins A and E Serum Controls
    Order no.: 0032/0036/0037
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  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
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  2. 34100 HPLC vitamin A vitamin E serum plasma analytical column
    HPLC column
    Order no.: 34100
    Vitamins A and E in Serum/Plasma
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  3. PEEK-encased Prefilter, 5 µm
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  4. Precolumn Cartridge Holder 4/10
    Details
  5. Precolumn cartridge 4/10
    Precolumn cartridge 4/10
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