MassChrom® Vitamins B1 and B6 in Whole Blood, with Reaction Vials

Order No.: 87000/RUO, for 480 tests
Parameters:
Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)

Fast and easy sample preparation
Robust method with high selectivity
Short run time of 2.5 minutes
Isotopically-labelled internal standard for each analyte
With reaction vials

Vitamin B1 (TPP, thiamine pyrophosphate)

Vitamin B6 (PLP, pyridoxal 5’-phosphate)

This assay enables combined analysis of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes. The chromatographic separation of the MassChrom® assay is fast but sufficiently resolving for a clear separation of the two analytes. Isotope- labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results.

The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.

More Information
Method of Analysis LC-MS/MS
Number of Tests 480
Analysis Time 2.5 min
Specimen Whole Blood
Sample Preparation
  • Pipette 40 μl research sample/calibrator/MassCheck® control into reaction vial.
  • Add 80 μl Internal Standard Mix, mix briefly (vortex).
  • Add 350 μl Precipitation Reagent, mix for 1 min (vortex) and centrifuge for 2 min.
  • Transfer supernatant into autosampler vial.
  • Inject 20 μl into the LC-MS/MS system.
Injection Volume 20 μl
Flow rate 0.5 ml/min
Column temperature 30 °C
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)
  1. Mobile Phase A
    Mobile Phase A
    Order no.: 87001/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 87002/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  3. Internal Standard Set Vitamins B1 and B6
    Internal Standard Set Vitamins B1 and B6
    Order no.: 87046/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  4. Precipitation Reagent
    Precipitation Reagent
    Order no.: 87005/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  5. Rinsing Solution
    Rinsing Solution
    Order no.: 87009/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  6. Reaction Vials, 2 ml
    Reaction Vials, 2 ml
    Order no.: 33008
    Details
  1. 87100 Analytical Column for Vitamins B1 and B6 in Whole Blood
    Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MS
    Order no.: 87100

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  1. 87100 Analytical Column for Vitamins B1 and B6 in Whole Blood
    Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MS
    Order no.: 87100

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  2. Tuning Mix Vitamins B1 and B6
    Tuning Mix Vitamins B1 and B6
    Order no.: 87015/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  3. Dilution Buffer
    Dilution Buffer
    Order no.: 87018/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details
  4. Adjustment Reagent
    Adjustment Reagent
    Order no.: 87019/RUO

    MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS

    Details