MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

Order No.: 62000/RUO & 62000/1000/RUO
Parameters:
25-OH-Vitamin D2, 25-OH-Vitamin D3

Suitable for every tandem-MS with sufficient sensitivity
Isotopically labelled internal standard
6PLUS1® and 3PLUS1® Multilevel Calibrator Sets
Online sample preparation
Minimal ion suppression effects

Grouped product items
Product Name Qty
MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma - LC-MS/MS
Order No.: 62000/RUO
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma (integrated kit for 1000 tests) - LC-MS/MS
Order No.: 62000/1000/RUO

25-OH-Vitamin D2

25-OH-Vitamin D3

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard, plus the multilevel calibrators (6PLUS1®, 3PLUS1®) assures high performance and minimises ion suppression effects.

An upgrade for the determination of the epimeric forms of 25-OH-Vitamin D3 and 25-OH-Vitamin D2 is also available for this kit (see accessories).

 

Kit content 62000/RUO                                

Mobile Phase A, 1000 ml                     
Mobile Phase B, 1000 ml                      
Precipitation Reagent, 5 ml                 
Internal Standard, 40 ml                        
Rinsing Solution, 1000 ml                    
Reaction vials, 2 x 100 pcs.        

 

Kit content 62000/1000/RUO   

Mobile Phase A, 5 x 1000 ml                                                                   
Mobile Phase B, 5 x 1000 ml                                                                  
Precipitation Reagent, 5 x 5 ml                                                                
Internal Standard, 5 x 40 ml                                                                    
Rinsing Solution, 2 x 1000 ml                                                        
Reaction vials, 10 x 100 pcs.        
Analytical column, 1 pc.           
Trap column, 2 pcs.                   
3PLUS1® 25-OH-Vitamin D3/D2 Multilevel Serum  Calibrator Set (lyoph.), 4 x 1 ml            
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml

More Information
Analysis Time 5 min
Sample Preparation
  • Pipette 100 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Precipitation Reagent.
  • Add 200 μl Internal Standard and mix 20 s (vortex)
  • Incubate 10 min at +4 °C.
  • Centrifuge 5 min at 15000 x g.
  • Transfer 200 μl supernatant into an autosampler vial.
  • Inject 10–50 μl of the supernatant into the HPLC-MS/MS system.
Sample Stability The prepared research samples are stable up to 4 days at room temperature and up to 1 week at +2 to +8 °C. For longer storage (maximum 2 weeks) deep-freeze the research samples below -18 °C.
Specimen Serum/Plasma
Injection Volume 10 to 50 μl (mass spectrometer dependent)
Ionisation APCI positive
MS/MS-Mode MRM
Additional Info

LC-MS/MS parameters

Information

The trap column and the analytical column are linked to the mass spectrometer through a 2-position 6-port valve.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 25-OH-Vitamin D2, 25-OH-Vitamin D3
Additional Info

HPLC parameters

Pump A delivers 100 % Mobile Phase A for loading the trap column.
Pump B delivers 100 % Mobile Phase A for separating analytes on the analytical column.

Flow

Pump A: 0.02–1.00 ml/min
Pump B: 0.60–1.00 ml/min

  1. Mobile Phase A
    Mobile Phase A
    Order no.: 62001/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  2. Mobile Phase B
    Mobile Phase B
    Order no.: 62002/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  3. Precipitation Reagent
    Precipitation Reagent
    Order no.: 62003/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  4. Internal Standard
    Internal Standard
    Order no.: 62004/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  5. Rinsing Solution
    Rinsing Solution
    Order no.: 62009/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  6. 3006 reaction vials
    Reaction Vials
    Order no.: 3006
    Details
  1. 0221 0222 0223 0256 Vitamin D Serum Controls
    MassCheck® 25-OH-Vitamin D3/D2 Serum Controls
    Order no.: 0221/0222/0223/0256
     
    Details
  1. Upgrade for 3-epi-25-OH-Vitamin D3/D2 - LC-MS/MS
    Upgrade for 3-epi-25-OH-Vitamin D3/D2 - LC-MS/MS
    Order no.: Epimer Upgrade/RUO

    Upgrade for order no. 62000/RUO

    Simultaneous analysis of
    – 25-OH-D3, 3-epi-25-OH-D3
    – 25-OH-D2, 3-epi-25-OH-D2
    2 isotopically labelled internal standards
    3PLUS1® Multilevel Calibrator Set and MassCheck® Controls
    Online sample preparation

    Details
  2. 62100 LCMS Vitamin D Analytical Column
    Analytical column
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  3. 62110 LCMS Vitamin D Trap Column
    Trap column
    Order no.: 62110

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  4. Tuning Mix 25-OH-Vitamin D3/D2
    Tuning Mix 25-OH-Vitamin D3/D2
    Order no.: 62015/RUO

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  5. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  6. PEEK-encased Prefilter, 2 µm
    Details