the Status Quo of Amino Acid Analysis in Plasma
Comparison of the Gold Standard Ion Exchange Chromatography with LC-MS/MS
Speaker: Rachel Carling, PhD, Consultant Scientist and Director of Biochemical Sciences, Viapath, UK
Date: Tuesday, September 29, 2020
Abstract: Quantitative amino acid analysis in human plasma is essential to the diagnosis and monitoring of inherited disorders of amino acid synthesis, catabolism and transport. Over the years, the gold standard has been to use Ion Exchange Chromatography (IEC). As with most techniques, IEC does have a number of drawbacks. Dr Rachel Carling from Viapath (UK) reports on a study that compares IEC with LC-MS/MS, a method with higher specificity and practical advantages. The data suggest that it might be time to rethink how we approach our quantitative amino acid analysis (AAA) experiments by using LC-MS/MS.