Vitamins A and E in Serum/Plasma - HPLC
2 specific precipitation steps
Low costs per test
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
- Specific method
- Easy sample preparation
- Low analysis costs
This established kit allows the simultaneous analysis of vitamins A and E in serum/plasma in a single isocratic HPLC run with UV detection. An easy sample preparation is initiating the effective protein precipitation step. A stable internal standard is used for precise quantification.
Chromsystems offers additional kits for the fast and efficient determination of vitamins A and E. You can choose between several options depending on the device capabilities (HPLC or UHPLC) and the sample quantities in your laboratory. Alternatively, the kit 34000/Premix with simplified sample preparation in premixed tubes is available, as well as the 34900/UHPLC kit for higher sample throughput and using UHPLC determination. Our kits are also available with automated workflow (34700/F and 34900/F).
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||
Vitamin A: 0.02 mg/l
Vitamin A: 0.02-5.0 mg/l
|Recovery||Vitamin A: 106 %; Vitamin E: 101 %|
Vitamin A: CV ≤ 2.5 %
Vitamin E: CV ≤ 2.4 %
Vitamin A: CV ≤ 5.0 %
Vitamin E: CV ≤ 4.9 %
|Analysis Time||approx. 9 min|
Time of sample drawing
Collection and storage of patient specimens
|Sample Stability||Protect prepared samples from light, at +2 to +8°C stable up to 12 hours.|
|Injection Volume||50 µl|
|Flow rate||1.5 ml/min|
|Column temperature||ambient (~ 25 °C)|
325 nm, after 3.5 min switch to 295 nm
|Additional Info||For the analysis any isocratic HPLC system with UV detector is suitable. For simultaneous analysis of the vitamins A and E, a detector with programmable wavelength switching is very useful as these vitamins are detected at different wavelengths.|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||Vitamin A, Vitamin E|