Urine Upgrade Set for MMA

Order No.: MMA Urine Set, for 200 tests
Parameters:
Methylmalonic Acid (MMA)

Upgrade for order no. 64000

MassChrom® Methylmalonic Acid

Grouped product items
Product Name Qty
3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid
Order No.: 64029
Dilution Buffer Urine
Order No.: 64007
Internal Standard Methylmalonic Acid in Urine
Order No.: 64044/U
Reaction Vials, transparent, 1.5 ml
Order No.: 33006
Methylmalonic Acid Urine Control, Bi-Level (I + II)
Order No.: 0316

Methylmalonic Acid (MMA)

Urine Upgrade Set for 200 Tests

The following items are additionally required to 64000:
64029 3PLUS1® Multilevel Urine Calibrator Set Methylmalonic Acid (lyoph.), 4 x 2 ml
64007 Dilution Buffer Urine, 75 ml
64004/U Internal Standard Methylmalonic Acid in Urine, 5 ml
33006 Reaction Vials, transparent, 100 pcs.
0317/0318 MassCheck® Methylmalonic Acid Urine Controls (lyoph.), 5 x 2 ml

More Information
Method of Analysis LC-MS/MS
Number of Tests 200
Limit of quantification

86 µg/l

Linearity

up to 9500 µg/l

Recovery 98 %
Intraassay

CV = 1.8-2.2 %

Interassay

CV = 2.4-2.9 %

Analysis Time 3.5 min
Specimen Urine
Sample Preparation
  • Pipette 200 µl sample/calibrator/control into transparent reaction vials
  • Add 25 µl Internal Standard and mix 10 s (vortex)
  • Dilute sample with Dilution Buffer in an autosampler vial
Sample Stability At +2 to +8 °C up to 14 days, below -18 °C up to 2 months.
Injection Volume 10 to 20 µl
Flow rate 0.7 ml/min
Column temperature ambient temperature (approx. 25 °C)
Gradient
0 min 100 % Mobile Phase A
2.5 min 0 % Mobile Phase A, 100 % Mobile Phase B
2.51-3.5 min 100 % Mobile Phase A
Ionisation ESI negative
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Methylmalonic Acid (MMA)
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