Parameter Set - Neuroleptics 2/EXTENDED - LC-MS/MS
Encompasses 13 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A
Amisulpride
Chlorprothixene
Levomepromazine
Melperone
Perazine
Pipamperone
Promethazine
Sertindole
Sulpiride
Thioridazine
Ziprasidone
Zotepine
Zuclopenthixol
Clinical relevance
Neuroleptic agents, also known as antipsychotics, are used to treat psychosis and other psychological disorders such as manic depressive conditions accompanied by delusions and hallucinations. Their effect is based on altering synaptic transmission in the central nervous system. The neuroleptics bind to specific receptors on the neurotransmitters such as dopamine, preventing transmission of stimulus information and inhibiting the transmission of neural signals.
To avoid interferences and enable a clear analysis, the neuroleptics were divided into two Parameter Sets, Neuroleptics 1/EXTENDED and Neuroleptics 2/EXTENDED 2. The present analysis set Neuroleptics 2/EXTENDED has been extended by the parameters melperone, sertindole, sulpiride, thioridazine, zotepine and zuclopenthixol.
Product advantages
With the Parameter Set Neuroleptics 2/EXTENDED in serum/plasma, 13 different analytes can be measured fast and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and reproducible and reliable quantification of the analytes. The method is comprehensively validated.
The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.
For the analysis you require the MassTox® TDM Basic Kit A, the specific MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.
| Method of Analysis | LC-MS/MS |
|---|---|
| Limit of quantification | 1 – 35 µg/l |
| Linearity | at least twice that of the therapeutic range |
| Recovery | 86 – 105 % |
| Intraassay | CV = 1.1 – 7.5 % |
| Interassay | CV = 3.1 – 7.7 % |
| Analysis Time | 3.5 min |
| Specimen | Serum/Plasma |
| Sample Preparation |
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| Sample Stability | Samples are stable for at least 24 hours when stored in the dark at +4°C. For longer storage periods keep samples frozen below -18 °C. |
| Injection Volume | 0.2 – 50 µl |
| Gradient | 0.00 - 0.20 min 0 % Mobile Phase 2 |
| Ionisation | ESI positive |
| MS/MS-Mode | MRM |
| Additional Info | We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width. |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Parameter | Amisulpride, Chlorprothixene, Levomepromazine, Melperone, Perazine, Pipamperone, Promethazine, Sertindole, Sulpiride, Thioridazine, Ziprasidone, Zotepine, Zuclopenthixol |
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Internal Standard Set Neuroleptics 2/EXTENDEDOrder no.: 92046/N2/XTComponent of the Parameter Set Neuroleptics 2/EXTENDED, available separately
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MassTox® TDM MasterColumn® AOrder no.: 92110Analytical column for MassTox® TDM Series A - LC-MS/MS
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Tuning Mix Neuroleptics 2/EXTENDEDOrder no.: 92017/XTTuning Mix for the Parameter Set Neuroleptics 2/EXTENDED - LC-MS/MS
