MassTox® Immunosuppressants in Whole Blood – ONEMinute Test for automated sample preparation - LC-MS/MS

Order No.: 93900/1200/DWP, for 1200 tests
Parameters:
Cyclosporin A, Everolimus, Sirolimus, Tacrolimus

96 Samples in approx. 45 minutes (liquid handling system dependent)
Suitable for many liquid handling devices
Separation by centrifugation
Fully validated automated workflow

Cyclosporin A

Everolimus

Sirolimus

Tacrolimus

Clinical relevance

Immunosuppressants suppress the activity of the immune system in order to prevent organ transplant rejection. They are also used as therapeutic drugs for the treatment of autoimmune diseases. Therapeutic drug monitoring of immunosuppressants is indispensable for therapy following after organ transplantation. Besides mycophenolic acid, the immunosuppressants most commonly used following an organ transplantion include cyclosporin A (INN: Ciclosporin), tacrolimus (FK506), sirolimus and everolimus. Cyclosporin A and tacrolimus block the cellular signal transduction, which is required for the immune response, by inhibiting the enzyme calcineurin. For instance, it controls the synthesis of interleukin in the T cells during an immune response. In contrast, sirolimus, and its derivative everolimus, are calcineurin-independent and inhibit mTOR (mammalian target of rapamycin), an important enzyme of the intracellular signal transduction during the immune response.

The quantitative determination of these immunosuppressants should be performed only with LC-MS/MS, because the immunological tests vary considerably in terms of selectivity and specificity, and sometimes also detect inactive metabolites. The compensation of matrix effects also plays a key role in the diagnostic analysis of immunosuppressants from a complex matrix such as whole blood. It is therefore essential that interferences are reduced significantly to achieve precise and reliable quantification.

 

Product advantages

  • Up to 800 samples per day
  • Reduced laboratory work required, higher throughput
  • Reliable elimination of matrix effects
  • High sensitivity and specificity
  • Very short analysis time of 1-2 minutes

The ONEMINUTE MassTox® assay for the analysis of the four immunosuppressants leads to robust, precise and reproducible results with a run time of only 1-2 minutes per sample. The ONEMINUTE method saves time, especially for laboratories with high sample throughput. Manual sample preparation is reduced to a simple and effective protein precipitation step. The concentration of the analytes and the elimination of other interfering substances is performed ONLINE using a trap column, which massively minimises the matrix effects. The isotopically labelled internal standards compensate entirely for remaining ion suppression. The trap column is connected by a simple switching system to the HPLC column and the MS/MS system. The additional use of 6PLUS1® multilevel calibrators and the MassCheck® controls ensures highly precise results.

This test is also available with manual sample preparation (order no. 93900) and as a standard method (order no. 93000).

More Information
Number of Tests 1200
Sample Preparation
  • Transfer 50 μl sample/calibrator/MassCheck® control into the Deep Well Extraction Plate.
  • Add 25 μl Internal Standard Mix
    + 100 μl Extraction Buffer
    + 250 μl Precipitation Reagent.
  • Agitate the extraction plate for 3 min.
  • Separate precipitate by centrifugation for 3 min at 2000 x g.
  • Transfer supernatant into the Collection Plate, seal it and transfer to autosampler for injection into the LC-MS/MS system.
Injection Volume 5–50 µl
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter Cyclosporin A, Everolimus, Sirolimus, Tacrolimus
  1. 93911 Mobile Phase A OneMinuteTest Immunosuppressants
    Mobile Phase A
    Order no.: 93911

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  2. 93122 Trap Column Immunosuppressants
    Mobile Phase B
    Order no.: 93922

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

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  3. 93003 Precipitation Reagent
    Precipitation Reagent
    Order no.: 93003

    MassTox® Immunosuppressants in Whole Blood - LC-MS/MS

    Details
  4. 93946 Internal Standard Mix Immunosuppressants
    Internal Standard Set
    Order no.: 93946

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  5. 93005 Extraction Buffer
    Extraction Buffer
    Order no.: 93005

    MassTox® Immunosuppressants in Whole Blood - LC-MS/MS

    Details
  6. 93909 LCMS MassTox Immunosuppressants Whole Blood OneMinute Rinsing Solution
    Rinsing Solution
    Order no.: 93909

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  7. 93956 Deep Well Extraction Plate Immunosuppressants
    96 Deep Well Extraction Plates
    Order no.: 93956

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  8. 93058_93059 OneMinute Collection Plate Seals
    Collection Plates
    Order no.: 93058

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  9. 93058_93059 OneMinute Collection Plate Seals
    Pierceable adhesive seals for 96 Well Plates
    Order no.: 93059

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  10. 28039/XL Immunosuppressants Multilevel Calibrator
    6PLUS1® Multilevel Whole Blood Calibrator Set Immunosuppressants
    Order no.: 28039/XL

    MassTox® Immunosuppressants in Whole Blood ONEMinute Test - LC-MS/MS

    Details
  11. 93100 Analytical Column Immunosuppressants
    Analytical column
    Order no.: 93100

    MassTox® Immunosuppressants in Whole Blood - LC-MS/MS

    Details
  12. 93122 Trap Column Immunosuppressants
    Trap column
    Order no.: 93122

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  1. 28039/XL Immunosuppressants Multilevel Calibrator
    6PLUS1® Multilevel Whole Blood Calibrator Set Immunosuppressants
    Order no.: 28039/XL

    MassTox® Immunosuppressants in Whole Blood ONEMinute Test - LC-MS/MS

    Details
  1. MassCheck® Immunosuppressants Whole Blood Controls
    MassCheck® Immunosuppressants Whole Blood Controls
    Order no.: 0081/0082/0083/0084/0085/0089

    MassTox® Immunosuppressants in Whole Blood - LC-MS/MS

    Details
  1. 93100 Analytical Column Immunosuppressants
    Analytical column
    Order no.: 93100

    MassTox® Immunosuppressants in Whole Blood - LC-MS/MS

    Details
  2. 93122 Trap Column Immunosuppressants
    Trap column
    Order no.: 93122

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  3. 93915 LCMS MassTox Immunosuppressants Whole Blood OneMinute Tuning Mix
    Tuning Mix Immunosuppressants ONEMinute Test
    Order no.: 93915

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  4. 93956 Deep Well Extraction Plate Immunosuppressants
    96 Deep Well Extraction Plates
    Order no.: 93956

    MassTox® Immunosuppressants ONEMinute Test - LC-MS/MS

    Details
  5. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
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