MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

Clinical relevance

Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

25-OH-vitamin D₃ is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)₂-vitamin D₃. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D₃ or vitamin D₂. Vitamin D₂ is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.

Product advantages

• Online sample preparation
• Isotopically labelled internal standard
• 3PLUS1® and 6PLUS1® Multilevel Calibrator Set and MassCheck® Controls
• Upgrade available for the determination of epimeric forms

This Chromsystems kit allows the specific determination of 25-OH-Vitamin D₃ and 25-OH-Vitamin D₂ in serum and plasma with LC-MS/MS. The manual sample preparation is limited to simple and effective protein precipitation. After this, the analytes are concentrated using an online trap column and interfering matrix components are separated. The trap column is connected by a simple switching system to the HPLC column, which provides further purification of the analytes. This method is characterised by the use of atmospheric pressure chemical ionisation (APCI). Isotopically labelled internal standards assure a high level of precision and robustness of the method and minimise matrix effects ("ion suppression"). The 3PLUS1® and 6PLUS1® multilevel calibrators are optionally available and guarantee a high level of precision of the results.

An upgrade for the determination of the epimeric forms of 25-OH-Vitamin D₃ and 25-OH-Vitamin D₂ is also available for this kit (see accessories), as well as a variant for automated sample preparation in 96 well filter plates (order no. 62000/1000/F).

Content for 200 tests (62000)
Mobile Phase A, 1000 ml
Mobile Phase B, 1000 ml
Precipitation Reagent, 5 ml
Internal Standard, 40 ml
Rinsing Solution, 1000 ml
Reaction Vials, 2 x 100 pcs. 

Content for 1000 tests (62000/1000)
Mobile Phase A, 5 x 1000 ml
Mobile Phase B, 5 x 1000 ml
Precipitation Reagent, 5 x 5 ml
Internal Standard, 5 x 40 ml
Rinsing Solution, 2 x 1000 ml
Reaction Vials, 10 x 100 pcs. 
3PLUS1® 25-OH-Vitamin D₃/D₂ Multilevel Serum Calibrator Set (lyoph.), 4 x 1 ml 
MassCheck® 25-OH-Vitamin D₃/D₂ Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck® 25-OH-Vitamin D₃/D₂ Serum Control, Level II (lyoph.), 5 x 1 ml 
Analytical Column, 1 pc. 
Trap Column, 2 pcs.