CDT in Serum - ternary gradient systems - HPLC

Order No.: 54030 & 54030/500
Parameters:
Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test
Reference method for CDT analyses: currently HPLC

Grouped product items
Product Name Qty
CDT in Serum - ternary gradient system - HPLC
Order No.: 54030
CDT in Serum - ternary gradient system - HPLC
Order No.: 54030/500

Asialotransferrin

Disialotransferrin

Pentasialotransferrin

Tetrasialotransferrin

Trisialotransferrin

Clinical relevance

Carbohydrate deficient transferrin (CDT) is regarded as the most specific laboratory marker of chronic alcohol abuse. Unlike otherbiochemical parameters such as γ-GT or MCV, false positive results caused by non-alcoholic liver diseases can be excluded. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a period of two weeks elevates CDT values significantly. After approx. 2 weeks of alcohol abstention, the levels normalise again. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic judgements or in occupational medicine.

 

Product advantages

  • Specific marker for chronic alcohol abuse
  • Long column lifetime
  • Traceable to the IFCC reference measurement procedure

This Chromsystems assay is designed for the fast and reliable determination of CDT in serum using HPLC with UV detection. The results are calculated as area percent of total transferrin, and, therefore, are not affected by variations of the total transferrin concentration. The optimised chromatographic separation of the transferrin isoforms also ensures the detection of genetic variants of transferrin.

This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore also be used to determine CDTIFCC. The IFCC certificate is available for customers in the secure download area.

Alternative methods using Pre-mixed Tubes or automated sample preparation for higher sample throughput are also available.

More Information
Limit of quantification approx. 0.5 % Disialotransferrin
Linearity up to at least 12 % Disialotransferrin
Recovery 99 %
Intraassay CV ≤ 6.2 %
Interassay CV ≤ 3.5 %
Analysis Time 20 min
Sample Preparation
  • Place 200 µl serum into a reaction vial.
  • Add 100 µl reaction mix*, mix for 60 s (vortex).
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at ≥ 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 μl each per sample)

Sample Stability Specimens should be kept cool for transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below –18°C.
Specimen Serum
Injection Volume 200 µl
Flow rate 1.5 ml/min
Column temperature ambient (~25 °C)
Wavelength 460 nm
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
Additional Info

For the Chromsystems HPLC analysis of CDT in serum any ternary HPLC gradient system with UV detection is suitable.

  1. 54031 HPLC mobile phase CDT serum
    Mobile Phase A
    Order no.: 54031
    CDT in Serum - ternary gradient systems - HPLC
    Details
  2. 54032 HPLC mobile phase CDT serum
    Mobile Phase B
    Order no.: 54032
    CDT in Serum - ternary gradient systems - HPLC
    Details
  3. 54033 HPLC CDT Mobile Phase C
    Mobile Phase C
    Order no.: 54033
    CDT in Serum - ternary gradient systems - HPLC
    Details
  4. 54025 HPLC neutralisation buffer CDT serum
    Neutralisation Buffer
    Order no.: 54025

    CDT in Serum - HPLC

    Details
  5. 54026 HPLC stabilisation buffer CDT serum
    Stabilisation Buffer
    Order no.: 54026
    CDT in Serum - HPLC
    Details
  6. 54027 HPLC precipitation reagent 1 CDT serum
    Precipitation Reagent 1
    Order no.: 54027
    CDT in Serum - HPLC
    Details
  7. 54028 HPLC precipitation reagent 2 CDT serum
    Precipitation Reagent 2
    Order no.: 54028

    CDT in Serum - HPLC

    Details
  8. 3006 reaction vials
    Reaction Vials
    Order no.: 3006
    Details
  1. 0168 0169 CDT serum controls
    CDT Serum Controls
    Order no.: 0168/0169
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. 54100 HPLC column CDT serum
    HPLC column
    Order no.: 54100

    CDT in Serum

    Details
  3. 54029 HPLC column wash buffer CDT serum
    Column Wash Buffer
    Order no.: 54029
    CDT in Serum - HPLC
    Details
  4. PEEK-encased Prefilter, 5 µm
    Details
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