CDT in Serum - binary gradient systems - HPLC

Order No.: 54020 & 54020/500
Parameters:
Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test
Reference method for CDT analyses: currently HPLC

Grouped product items
Product Name Qty
CDT in Serum - binary gradient system - HPLC
Order No.: 54020
CDT in Serum - binary gradient system - HPLC
Order No.: 54020/500

Asialotransferrin

Disialotransferrin

Pentasialotransferrin

Tetrasialotransferrin

Trisialotransferrin

Clinical relevance:

Carbohydrate deficient transferrin (CDT) is regarded as the most specific laboratory marker of chronic alcohol abuse. Unlike other biochemical parameters such as γ-GT or MCV, false positive results caused by non-alcoholic liver diseases can be excluded. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a period of two weeks elevates CDT values significantly. After approx. 2 weeks of alcohol abstention, the levels normalise again. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic judgements or in occupational medicine.

 

Product advantages:

  • Specific marker of chronic alcohol abuse
  • Long column lifetime
  • Traceable to the IFCC reference measurement procedure (ternary method)


This Chromsystems assay is designed for the fast and reliable determination of CDT in serum using HPLC with UV detection. The results are calculated as area percent of total transferrin, and, therefore, are not affected by variations of the total transferrin concentration. The optimised chromatographic separation of the transferrin isoforms also ensures the detection of genetic variants of transferrin.

More Information
Method of Analysis HPLC
Limit of quantification approx. 0.5 % Disialotransferrin
Linearity up to at least 12 % Disialotransferrin
Intraassay CV ≤ 7.7 %
Interassay

CV ≤ 4.6 %

Analysis Time 22 min
Specimen Serum
Sample Preparation
  • Place 200 µl sample/serum into a reaction vial.
  • Add 100 µl reaction mix*, mix for 60 s (vortex).
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at ≥ 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 μl each per sample)

Sample Stability Specimens should be kept cool for transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below –18°C.
Injection Volume 200 µl
Flow rate 1.5 ml/min
Column temperature ambient (~25 °C)
Wavelength 460 nm
Additional Info For the Chromsystems HPLC analysis of CDT in serum any binary HPLC gradient system with UV detection is suitable.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin
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