Drugs of Abuse Testing

The CE-IVD validated solution includes everything, eliminating the need for developing and maintaining several in-house methods or for preparing QC materials for a multitude of analyte compounds. It also provides a significantly higher selectivity and sensitivity than current immunoassays.

  • Assay includes 108 drugs and metabolites
  • Target Screening and Quantitative Confirmation within the same run
  • Run time of 12 minutes
  • Simple sample preparation combined with high selectivity and sensitivity
  • Internal standards for 98 analytes 

Parameter Menu of 108 Drugs

Amphetamine, BDB, Butylone, 2C-B, 2C-I, Cathinone, MBDB, MDA, MDEA, MDMA, MDPV, Mephedrone, Methamphetamine, Methaqualone, Methylone, Methylphenidate, PMA, Ritalinic Acid

Allobarbital, Amobarbital, Barbital, Butalbital, Hexobarbital, Pentobarbital, Phenobarbital, Secbutabarbital, Secobarbital, Thiopental

Alprazolam, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 7-Aminonitrazepam, Bromazepam, Brotizolam, Chlordiazepoxide, Clobazam, Clonazepam, Demoxepam, Desalkylflurazepam, Desmethylflunitrazepam, Diazepam, Estazolam, Flunitrazepam, Flurazepam, Lorazepam, Lormetazepam, Medazepam, Midazolam, Nitrazepam, Norclobazam, Nordiazepam, α-OH-Alprazolam, 3-OH-Bromazepam, α-OH-Midazolam, α-OH-Triazolam, Oxazepam, Prazepam, Temazepam, Triazolam

Gabapentin, Pregabalin, Promethazine, Quetiapine


Benzoylecgonine, Cocaethylene, Cocaine, Norcocaine

Acetylcodeine, Buprenorphine, Codeine, Dihydrocodeine, EDDP, Fentanyl, Hydrocodone, Hydromorphone, Meconin, Meperidine, Methadone, 6-Monoacetylmorphine, Morphine, Naloxone, Naltrexone, Norbuprenorphine, Norcodeine, Norfentanyl, Normeperidine, Nortapentadol, Nortilidine, O-Desmethyltramadol, Oxycodone, Oxymorphone, Papaverine, Propoxyphene, Sufentanil, Tapentadol, Thebaine, Tilidine, Tramadol

Zaleplon, Zolpidem, Zopiclone

Ketamine, LSD, Mescaline, Norketamine, O-H-LSD, PCP

2C-B, 2C-I, 3-OH-Bromazepam, 6-Monoacetylmorphine, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 7-Aminonitrazepam, Acetylcodeine, Allobarbital, Alprazolam, Amobarbital, Amphetamine, Barbital, BDB, Benzoylecgonine, Bromazepam, Brotizolam, Buprenorphine, Butalbital, Butylone, Cathinone, Chlordiazepoxide, Clobazam, Clonazepam, Cocaethylene, Cocaine, Codeine, Demoxepam, Desalkylflurazepam, Desmethylflunitrazepam, Diazepam, Dihydrocodeine, EDDP, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Gabapentin, Hexobarbital, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lormetazepam, LSD, MBDB, MDA, MDEA, MDMA, MDPV, Meconin, Medazepam, Meperidine, Mephedrone, Mescaline, Methadone, Methamphetamine, Methaqualone, Methylone, Methylphenidate, Midazolam, Morphine, Naloxone, Naltrexone, Nitrazepam, Norbuprenorphine, Norclobazam, Norcocaine, Norcodeine, Nordiazepam, Norfentanyl, Norketamine, Normeperidine, Nortapentadol, Nortilidine, O-Desmethyltramadol, O-H-LSD, Oxazepam, Oxycodone, Oxymorphone, Papaverine, PCP, Pentobarbital, Phenobarbital, PMA, Prazepam, Pregabalin, Promethazine, Propoxyphene, Quetiapine, Ritalinic Acid, Secbutabarbital, Secobarbital, Sufentanil, Tapentadol, Temazepam, THC-COOH, Thebaine, Thiopental, Tilidine, Tramadol, Triazolam, Zaleplon, Zolpidem, Zopiclone, α-OH-Alprazolam, α-OH-Midazolam, α-OH-Triazolam

100% Hydrolysis – 0% Doubt

Many drugs undergo glucuronidation prior to excretion, which requires an enzymatic or acidic hydrolysis – a challenge for most assays and in-house methods. In contrast, the MassTox® Drugs of Abuse Testing assay from Chromsystems includes a carefully selected enzyme that has proven to function with all drugs, including codeine and other substances usually difficult to hydrolyse. This ensures a complete and selective hydrolysis of all drugs covered in the assay.

Proven Accuracy and Reliability

A lab experienced in routine drugs of abuse testing performed a comparative analysis of the MassTox® assay with an LC-MS/MS based inhouse method. Values obtained correspond very well with each other at concentrations within the linearity of the method. This demonstrates the accuracy of data and the suitability of the Chromsystems assay to replace LC-MS/MS based in-house methods in the forensic and clinical laboratory. The MassTox® assay also has been successfully used in proficiency testing schemes of GTFCh, Instand and RfB. 

LC-MS/MS: Faster Sample Prep – Lower Workload

A comparison of the utility of the LC-MS/MS assay in the daily routine with GC-MS also has been made. Results not surprisingly demonstrate that LC-MS/MS allows the direct analysis of the specimens and eliminates the need for derivatisation steps. This makes the sample prep much simpler and less time-consuming in comparison to GC-MS. Another advantage is the need for much lower sample volumes. This means: when comparing our LC-MS/MS assay with GC-MS, then it provides comparable data, but with the advantage for LC-MS/MS to offer a faster sample prep and lower sample volumes.

Total run time 12 min 10-15 min
Sample prep time (excluding hydrolysis) 2 h per day 6 h per day
Sample volume 50 μl 1000 - 5000 μl



Assays for Drugs of Abuse

MassTox® Drugs of Abuse Testing in Urine

Order No. Method Number of tests
96000 LC-MS/MS 400 With reaction vials
96000/WP LC-MS/MS 400 With 96 well plates


Download Brochure

You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.

Drugs of Abuse Brochure ChromsystemsDrugs of Abuse Brochure Chromsystems

More about Drugs of Abuse Testing

In this article we compare LC-MS/MS with GC-MS currently considered as gold standard. The benefits of LC-MS/MS are demonstrated and it is shown how a 100% hydrolysis of glucuroniated drugs can be achieved.

Drugs of abuse testing from screening to confirmation with speaker Katja Ackermann, MVZ Labor 28 (Berlin) and Dr Christoph Geffert from Labor Staber Dresden-Klipphausen.

Chromsystems Workshop at TIAFT 2019 in Birmingham - with Dr Lewis Couchman from Analytical Services International in London and Dr Christoph Geffert from Labor Staber Dresden-Klipphausen.