Parameter Set Antidepressants 2/ Psychostimulants/EXTENDED - LC-MS/MS

Order No.: 92915/XT
Parameters:
Atomoxetine, Bupropion, Clomethiazole, Dosulepin, erythro-Dihydrobupropion, Hydroxybupropion, Methylphenidate, Mianserin, Milnacipran, Moclobemide, N-Desmethyldosulepin, Opipramol, Reboxetine, Ritalinic Acid, threo-Dihydrobupropion, Tianeptine, Tranylcypromine, Trazodone, Vilazodone, Vortioxetine

Expanded parameter menu of 20 analytes
Additional internal standards for enhanced robustness
Part of the continuously updated MassTox® TDM Series A

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Atomoxetine

Bupropion

erythro-Dihydrobupropion

Hydroxybupropion

threo-Dihydrobupropion

Clomethiazole

Dosulepin

N-Desmethyldosulepin

Methylphenidate

Mianserin

Milnacipran

Moclobemide

Opipramol

Reboxetine

Ritalinic acid

Tianeptine

Tranylcypromine

Trazodone

Vilazodone

Vortioxetine

Clinical relevance

Antidepressants are a group of chemically different drugs used to treat depression, obsessive-compulsive disorder, anxiety disorders and chronic pain. They modify the metabolism of the body's own neurotransmitters, primarily those of serotonin and norepinephrine, or react with their receptors.

Psychostimulants increase the activity of the central nervous system. Among other things, they eliminate fatigue, reduce the need for sleep, increase the ability to concentrate and inhibit the appetite.
This parameter set also includes psychostimulants such as methylphenidate (Ritalin®). The amphetamine-like substance is mainly used in the treatment of attention deficit/hyperactivity disorder (ADHD). Methylphenidate inhibits the reuptake of dopamine and noradrenaline, thus increasing their concentration in the synaptic cleft and enhancing the activity of the sympathetic nervous system. Atomoxetine, which has a chemical structure similar to fluoxetine, is another drug used to treat ADHD. In Germany, atomoxetine is approved for ADHD and is not subject to the controlled-substances law of Germany (Betäubungsmittelgesetz BtMG). Mianserin, reboxetine, and trazodone are prescribed for depression.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

 

MassSTAR

For this parameter set a CE-IVD compliant workflow method is also available using the MassSTAR to automate the sample preparation.

More information about MassSTAR

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.01 – 115 μg/l
Upper Limit of Quantification up to 150 – 12.500 μg/l
Intraassay CV = 2.0 – 13.7 %
Interassay CV = 5.3 – 13.2 %
Recovery 81 – 113%
Sample Preparation
  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time Group 1 = 2.8 min, Group 2 = 6 min
Injection Volume 0.2 – 50 µl
Gradient Group 1 binary, Group 2 isocratic
Ionisation ESI positive
MS/MS Mode Group 1 sMRM, Group 2 MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Atomoxetine, Bupropion, Clomethiazole, Dosulepin, erythro-Dihydrobupropion, Hydroxybupropion, Methylphenidate, Mianserin, Milnacipran, Moclobemide, N-Desmethyldosulepin, Opipramol, Reboxetine, Ritalinic Acid, threo-Dihydrobupropion, Tianeptine, Tranylcypromine, Trazodone, Vilazodone, Vortioxetine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Atomoxetine

Bupropion

erythro-Dihydrobupropion

Hydroxybupropion

threo-Dihydrobupropion

Clomethiazole

Dosulepin

N-Desmethyldosulepin

Methylphenidate

Mianserin

Milnacipran

Moclobemide

Opipramol

Reboxetine

Ritalinic acid

Tianeptine

Tranylcypromine

Trazodone

Vilazodone

Vortioxetine

Clinical relevance

Antidepressants are a group of chemically different drugs used to treat depression, obsessive-compulsive disorder, anxiety disorders and chronic pain. They modify the metabolism of the body's own neurotransmitters, primarily those of serotonin and norepinephrine, or react with their receptors.

Psychostimulants increase the activity of the central nervous system. Among other things, they eliminate fatigue, reduce the need for sleep, increase the ability to concentrate and inhibit the appetite.
This parameter set also includes psychostimulants such as methylphenidate (Ritalin®). The amphetamine-like substance is mainly used in the treatment of attention deficit/hyperactivity disorder (ADHD). Methylphenidate inhibits the reuptake of dopamine and noradrenaline, thus increasing their concentration in the synaptic cleft and enhancing the activity of the sympathetic nervous system. Atomoxetine, which has a chemical structure similar to fluoxetine, is another drug used to treat ADHD. In Germany, atomoxetine is approved for ADHD and is not subject to the controlled-substances law of Germany (Betäubungsmittelgesetz BtMG). Mianserin, reboxetine, and trazodone are prescribed for depression.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

 

MassSTAR

For this parameter set a CE-IVD compliant workflow method is also available using the MassSTAR to automate the sample preparation.

More information about MassSTAR

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.01 – 115 μg/l
Upper Limit of Quantification up to 150 – 12.500 μg/l
Intraassay CV = 2.0 – 13.7 %
Interassay CV = 5.3 – 13.2 %
Recovery 81 – 113%
Sample Preparation
  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time Group 1 = 2.8 min, Group 2 = 6 min
Injection Volume 0.2 – 50 µl
Gradient Group 1 binary, Group 2 isocratic
Ionisation ESI positive
MS/MS Mode Group 1 sMRM, Group 2 MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Atomoxetine, Bupropion, Clomethiazole, Dosulepin, erythro-Dihydrobupropion, Hydroxybupropion, Methylphenidate, Mianserin, Milnacipran, Moclobemide, N-Desmethyldosulepin, Opipramol, Reboxetine, Ritalinic Acid, threo-Dihydrobupropion, Tianeptine, Tranylcypromine, Trazodone, Vilazodone, Vortioxetine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.